Strain Data Sheet

RBRC04798

Strain Information

Image
BRC No.RBRC04798
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd
Former Common nameNDE-Pkd2/Venus Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Satoko Yoshiba and Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2008. Transgene was injected into the pronuclei of B6C3F1 fertilized eggs.
Strain descriptionThis transgenic mouse strain expresses the Pkd2 and venus (a variant of green fluorescent protein) fusion gene on both node under the control of the mouse node-specific enhancer.
Colony maintenanceCarrier X Noncarrier [C57BL/6JJcl]
References
Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2.
Yoshiba S, Shiratori H, Kuo I Y, Kawasumi A, Shinohara K, Nonaka S, Asai Y, Sasaki G, Belo J A, Sasaki H, Nakai J, Dworniczak B, Ehrlich B E, Pennekamp P, Hamada H
Science, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pkd2 polycystic kidney disease 2UNPkd2 mouse node-specific enhancer, hsp promoter
Venus Yellow Fluorescent Protein (Aequorea victoria)UNVenus

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Nodal enhancer, mouse Hsp68(Hspa1a) promoter, mouse Pkd2 cDNA, Jellyfish venus cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 338, 226-231 (2012). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data