RBRC04786

Information1

Image
BRC No.RBRC04786
TypeTransposon insertionCartagena
SpeciesMus musculus
Strain nameB6;B6D2(ICR)-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>
Former Common nameB6;B6D2(ICR)-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Drs. Junji Takeda (Oasaka University) and Hirotomo Saitsu (Yokohama City University)
Strain descriptionSMOC1 gene-traped mice that have an EGFP in Smoc1 gene, produced by using the SB transposon system. SMOC1 is essential for ocular and limb development. Homozygous mutant mice die within 3 weeks after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesNat. Methods, 2, 763-769 (2005). 16179923
Am. J. Hum. Genet., 88, 30-41 (2011). 21194678

Health Report

Examination Date / Room / Rack2012/07/23Room:4-ARack:G
2012/05/21Room:4-ARack:G
2012/03/26Room:4-ARack:G
2012/01/23Room:4-ARack:G
2011/11/28Room:4-ARack:G
2011/10/19Room:4-ARack:G
2011/10/17Room:4-ARack:G
2011/10/17Room:4-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Smoc1SPARC related modular calcium binding 112Smoc1SPARC related modular calcium binding 1

Information2

Donor DNASalmon Sleeping Beauty transposon, Salmon Sleeping Beauty transposase, Jellyfish GFP gene, E. coli LacZ gene, E. coli neo gene, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human bc1-2 splice acceptor, Mouse hprt splice donor, Cytomegalovirus enhancer, Chicken beta-actin promoter, Mouse pgk promoter, Rabbit beta-globin adenylation signal sequence, Mouse synaptonemal protein 1 promoter, Human growth hormone poly adenylation signal sequence, pBluescript
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005). Am. J. Genet., 88, 30-41 (2011).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Takeda Lab HP

BRC mice in Publications

No Data