Strain Data Sheet

RBRC04786

Strain Information

Image
BRC No.RBRC04786
TypeTransposon insertionCartagena
SpeciesMus musculus
Strain nameB6;B6D2(ICR)-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>
Former Common nameB6;B6D2(ICR)-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Drs. Junji Takeda (Oasaka University) and Hirotomo Saitsu (Yokohama City University)
Strain descriptionSMOC1 gene-traped mice that have an EGFP in Smoc1 gene, produced by using the SB transposon system. SMOC1 is essential for ocular and limb development. Homozygous mutant mice die within 3 weeks after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Vincent W Keng, Kojiro Yae, Tomoko Hayakawa, Sumi Mizuno, Yoshihiro Uno, Kosuke Yusa, Chikara Kokubu, Taroh Kinoshita, Keiko Akagi, Nancy A Jenkins, Neal G Copeland, Kyoji Horie, Junji Takeda
Nat. Methods, 2, 763-769 (2005). 16179923

SMOC1 is essential for ocular and limb development in humans and mice.
Ippei Okada, Haruka Hamanoue, Koji Terada, Takaya Tohma, Andre Megarbane, Eliane Chouery, Joelle Abou-Ghoch, Nadine Jalkh, Ozgur Cogulu, Ferda Ozkinay, Kyoji Horie, Junji Takeda, Tatsuya Furuichi, Shiro Ikegawa, Kiyomi Nishiyama, Satoko Miyatake, Akira Nishimura, Takeshi Mizuguchi, Norio Niikawa, Fumiki Hirahara, Tadashi Kaname, Koh-Ichiro Yoshiura, Yoshinori Tsurusaki, Hiroshi Doi, Noriko Miyake, Takahisa Furukawa, Naomichi Matsumoto, Hirotomo Saitsu
Am. J. Hum. Genet., 88, 30-41 (2011). 21194678

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Smoc1
MGI:1929878		SPARC related modular calcium binding 112Smoc1 SPARC related modular calcium binding 1
  • microphthalmia with limb anomalies(DisGeNET, MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal hindlimb morphology (MP:0000556)

  • abnormal interdigital cell death (MP:0009874)

  • abnormal joint morphology (MP:0002932)

  • abnormal pollex morphology (MP:0010772)

  • abnormal retina morphology (MP:0001325)
    • more 20 phenotypes
  • abnormal retina pigment epithelium morphology (MP:0005201)

  • absent optic nerve (MP:0001333)

  • bowed tibia (MP:0004358)

  • cleft palate (MP:0000111)

  • clubfoot (MP:0000576)

  • decreased body size (MP:0001265)

  • decreased body weight (MP:0001262)

  • decreased retina ganglion cell number (MP:0006309)

  • fused metatarsal bones (MP:0004642)

  • high palate (MP:0003757)

  • interdigital webbing (MP:0000571)

  • microphthalmia (MP:0001297)

  • optic nerve hypoplasia (MP:0006221)

  • pes valgus (MP:0031291)

  • postnatal growth retardation (MP:0001732)

  • postnatal lethality, complete penetrance (MP:0011085)

  • retina degeneration (MP:0001326)

  • short fibula (MP:0002765)

  • syndactyly (MP:0000564)

  • synostosis (MP:0000566)
    • Detailed phenotype data

    Ordering Information

    Donor DNASalmon Sleeping Beauty transposon, Salmon Sleeping Beauty transposase, Jellyfish GFP gene, E. coli LacZ gene, E. coli neo gene, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human bc1-2 splice acceptor, Mouse hprt splice donor, Cytomegalovirus enhancer, Chicken beta-actin promoter, Mouse pgk promoter, Rabbit beta-globin adenylation signal sequence, Mouse synaptonemal protein 1 promoter, Human growth hormone poly adenylation signal sequence, pBluescript
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005). Am. J. Genet., 88, 30-41 (2011).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data