Strain Data Sheet

RBRC04720

Strain Information

Image
BRC No.RBRC04720
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Rap1a<tm1Morz> Rap1b<tm1Morz>
Former Common nameB6-Rap1a/Rap1b floxed
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentNational Institute of Mental Health・Dr. Alexei Morozov.
Strain descriptionRap1a/Rap1b遺伝子のfloxedマウス。Rap1a遺伝子のエクソン2-3とRap1b遺伝子のエクソン1がloxPで挟まれている。
Colony maintenanceRap1a : Homozygote x Homozygote ; Rap1b : Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesJ. Neurosci., 28, 2089-2098 (2008). 18305243

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rap1bRAS-related protein-1b10Rap1b<tm1Morz>targeted mutation 1, Alexei Morozov

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rap1aRAS-related protein-1a3Rap1a<tm1Morz>targeted mutation 1, Alexei Morozov

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

供与核酸mouse Rapla genomic DNA, mouse Raplb genomic DNA, herpers simplex thymidine kinase promoter, E. coli Neomycin resistance gene, Bacteriophage P1 loxP site
Research applicationCre/loxP system
提供条件条件を付加する。
a) In all RECIPIENT publications related to the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested:
J Neurosci. 2008 Feb 27;28(9):2089-98.Enhanced cortico-amygdala efficacy and suppressed fear in absence of Rap1. Pan BX, Vautier F, Ito W, Bolshakov VY, Morozov A.
b) In all publications or presentations of any research results obtained by use of the BIOLOGICAL RESOURCE, RECIPIENT will acknowledge the DEPOSITOR as the source of the BIOLOGICAL RESOURCE.
c) Use of the BIOLOGICAL RESOURCE for commercial purposes will require a license agreement between NIH and RECIPIENT. Information can be obtained at https://www.ott.nih.gov./.
d) Prior to filing an application for a patent, or intellectual property or other rights based on the use of the BIOLOGICAL RESOURCE, RECIPIENT will first notify the DEPOSITOR.
e) RECIPIENT agrees not to claim, infer, or imply endorsement by the DEPOSITOR.
f) Unless prohibited by law from doing so, RECIPIENT agrees to hold the DEPOSITOR harmless and to indemnify the DEPOSITOR for all liabilities, demands, damages, expenses and losses arising out of RECIPIENT 's use for any purpose of the BIOLOGICAL RESOURCE.
g) This BIOLOGICAL RESOURCE is provided as a service to the research community. IT IS BEING SUPPLIED TO RECIPIENT WITH NO WARRANTIES, EXPRESS OR IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. RECIPIENT acknowledges that DEPOSITOR makes no representations that the use of the BIOLOGICAL RESOURCE will not infringe any patent or proprietary rights of third parties.
DepositorAlexei Morozov（National Institutes of Health）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Nagai T, Nakamuta S, Kuroda K, Nakauchi S, Nishioka T, Takano T, Zhang X, Tsuboi D, Funahashi Y, Nakano T, Yoshimoto J, Kobayashi K, Uchigashima M, Watanabe M, Miura M, Nishi A, Kobayashi K, Yamada K, Amano M, Kaibuchi K.
Phosphoproteomics of the Dopamine Pathway Enables Discovery of Rap1 Activation as a Reward Signal In Vivo.
Neuron 89(3) 550-65(2016) 26804993