Strain Data Sheet

RBRC04707

Strain Information

Image
BRC No.RBRC04707
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Kcnc1-EYFP)27Bsi Gad1<tm1Tama>
Former Common nameKv3.GYW
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development理化学研究所脳科学総合研究センター・Dr. Thomas Knoepfel。
Strain descriptionKcnc1-YFPトランスジェニックとGAD67 GFPノックインのダブルミュータントマウス。Kcnc1-YFP TgはラットKv3.1 potassium channelの5kb 5’ regulatory sequence制御下でYFPを発現する。GAD67 GFP KIはGAD67遺伝子のエクソン1のATGにGFPが挿入されている。GAD67 GFP KIホモマウスは繁殖不可。
Colony maintenanceTg : Carrier x Carrier; KO : Heterozygote x Wild-type
References
Transgenic mice expressing a pH and Cl- sensing yellow-fluorescent protein under the control of a potassium channel promoter.
Metzger F, Repunte-Canonigo V, Matsushita S, Akemann W, Diez-Garcia J, Ho C S, Iwasato T, Grandes P, Itohara S, Joho R H, Knopfel T
Eur. J. Neurosci., 15, 40-50 (2002). 11860505

Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse.
Tamamaki N, Yanagawa Y, Tomioka R, Miyazaki J, Obata K, Kaneko T
J. Comp. Neurol., 467, 60-79 (2003). 14574680

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)2
Gad1
MGI:95632		glutamate decarboxylase 12Gad1<tm1Tama>
MGI:3590300		targeted mutation 1, Nobuaki Tamamaki
  • developmental and epileptic encephalopathy 89(MedGEN)
    • loxP phage P1 loxP2loxP
    EYFP Enhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP rat Kcnc1 promoter

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal involuntary movement (MP:0003492)

  • abnormal nervous system electrophysiology (MP:0002272)
    • Detailed phenotype data

    Ordering Information

    供与核酸rat Kv3. 1 (Kcnc1) genomic DNA, Jellyfish EYFP cDNA, Simian virus SV40 polyA, mouse GAD67 genimic DNA, Jellyfish GFP cDNA, Phage P1 loxP, [Mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene ]
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    提供条件BEFORE the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
    a. The RECIPIENT shall acquire a prior written consent from the DEPOSITOR using the Approval form.
    b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    c. The RECIPIENT shall acquire and a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    d. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested, Eur J Neurosci. 2002 15(1):40-50., J Comp Neurol. 2003 467(1):60-79.
    AFTER the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
    a. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested, (will be announced).
    b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorThomas Knöepfel（独立行政法人理化学研究所）
    Strain Status凍結精子のアイコン凍結精子
    Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data