Strain Data Sheet

RBRC04707

Strain Information

Image
BRC No.RBRC04707
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Kcnc1-EYFP)27Bsi Gad1<tm1Tama>
Former Common nameKv3.GYW
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development理化学研究所脳科学総合研究センター・Dr. Thomas Knoepfel。
Strain descriptionKcnc1-YFPトランスジェニックとGAD67 GFPノックインのダブルミュータントマウス。Kcnc1-YFP TgはラットKv3.1 potassium channelの5kb 5’ regulatory sequence制御下でYFPを発現する。GAD67 GFP KIはGAD67遺伝子のエクソン1のATGにGFPが挿入されている。GAD67 GFP KIホモマウスは繁殖不可。
Colony maintenanceTg : Carrier x Carrier; KO : Heterozygote x Wild-type
ReferencesJ. Comp. Neurol., 467, 60-79 (2003). 14574680
Eur. J. Neurosci., 15, 40-50 (2002). 11860505

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gad1glutamate decarboxylase 12Gad1<tm1Tama>targeted mutation 1, Nobuaki Tamamaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFPrat Kcnc1 promoter

Ordering Information

供与核酸rat Kv3. 1 (Kcnc1) genomic DNA, Jellyfish EYFP cDNA, Simian virus SV40 polyA, mouse GAD67 genimic DNA, Jellyfish GFP cDNA, Phage P1 loxP, [Mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene ]
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
BEFORE the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
a. The RECIPIENT shall acquire a prior written consent from the DEPOSITOR using the Approval form.
b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
c. The RECIPIENT shall acquire and a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
d. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested, Eur J Neurosci. 2002 15(1):40-50., J Comp Neurol. 2003 467(1):60-79.
AFTER the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
a. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested, (will be announced).
b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorThomas Knöepfel（独立行政法人理化学研究所）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Contact Information
Genotyping protocol -PCR-

BRC mice in Publications

No Data