Strain Data Sheet

RBRC04703

Strain Information

Image
BRC No.RBRC04703
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Pih1d3<tm1Hmd>
Former Common nameTwister (flox/+)
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain development大阪大学大学院生命機能研究科・濱田博司先生（2006）。G4 ES細胞を用いて作出。CAG-FLPeマウスとの交配により、PGK-neo配列を除去。
Strain descriptionPih1d3 (Stp/Twister) 遺伝子のfloxedマウス。Pih1d3の終止コドンの直前にIRES-lacZが挿入されており、遺伝子の発現をlacZ発現でトレース可能。
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ. Cell Biol., 204, 203-213 (2014). 24421334

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pih1d3PIH1 domain containing 31Pih1d3<tm1Hmd>targeted mutation 1, Hiroshi HamadaSfp; sperm flagellar protein

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site1Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)1internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
myc-tagmyc-epitope tag1myc-tag

Ordering Information

供与核酸phage P1 loxP, yeast FRT (flipase recombination target) site, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, Myc-tag, mouse Pih1d3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって譲渡者の指定する文献を引用する。J. Cell Biol., 204, 203-213 (2014).
Depositor濱田　博司（国立研究開発法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data