Strain Information | |
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Image | |
BRC No. | RBRC04436 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Msi2<tm1Okn> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Shinsuke Shibata and Hideyuki Okano, Keio University School of Medicine. |
Strain description | Msi2 deficient miceMsi2 deficient mice survive to adult and show poor weight gain, low spontaneous movement and sensory dysfunction, with abnormal PNS development. The cause of the phenotype is thought to be poor neural fiber innervations of specific sensory neuron from DRG to spinal cord. These phenotypes are observed both background (C57BL/6J and CD-1) and detailed analyses were performed in CD-1 background.Msi2 deficient male mice show azoospermia and are infertile. Because the movement of sperm taken from epidermis of Msi2 null male was bad, in vitro fertilization was not successful. Msi2 deficient female mice are fertile; however, probability of pregnancy is poor presumably due to the low spontaneous activity. (All date of Msi2 deficient mice are in preparation by Shibata S. et al) |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin; Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ApolyA11poly A |
Ordering Information | |
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供与核酸 | mouse Musashi2 genomic DNA, E. coli Tn5neo gene, mouse PGK promoter, SV40 PolyA |
Research application | Cell Biology Research Neurobiology Research |
提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。(will be announced.) 利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 |
Depositor | 岡野 栄之(慶應義塾大学) |
Strain Status | 凍結精子 |
Strain Availability | 凍結精子より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |