Strain Data Sheet

RBRC04435

Strain Information

Image
BRC No.RBRC04435
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.129(B6)-Msi1<tm1Okn>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shinsuke Shibata and Hideyuki Okano, Keio University School of Medicine.
Strain descriptionMsi1 deficient miceC57BL/6J background Msi1 deficient mice frequently died of obstructive hydrocephalus within a couple of month with aberrant cell proliferation around cerebral aqueduct. (Sakakibara S et al. Proc Natl Acad Sci USA. 2002) Both male and female are infertile because of the early death of hydrocephalus.On the other hand, CD-1 (ICR) background Msi1 deficient mice survive to adult without gross anatomical phenotype. Since both male and female are fertile, it is possible to mate between Msi1 null mice. (Unpublished data)
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 99, 15194-15199 (2002). 12407178

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Msi1Musashi homolog 1 (Drosophila)5Msi1

Ordering Information

供与核酸mouse Musashi1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), PGK polyA signal
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 99, 15194-15199 (2002).
利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
Depositor岡野　栄之（慶應義塾大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Wolfe AR, Ernlund A, McGuinness W, Lehmann C, Carl K, Balmaceda N, Neufeld KL.
Suppression of intestinal tumorigenesis in Apc mutant mice upon Musashi-1 deletion.
J Cell Sci 130(4) 805-813(2017) 28082422