Strain Data Sheet

RBRC04420

Strain Information

Image
BRC No.RBRC04420
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(SJL)-Tcirg1<tm1.1Ywd>
Former Common namea3-GFP, Tcirg1<floxGFP>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development開発者 / 機関 : 大阪大学開発年 : 2008ES cell : R1 (129X1/SvJ x 129S1/Sv)F1-Kitl<+>C57Bl/6
Strain descriptionThis allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. The modified gene encodes a fusion protein in which the C-terminal of TCIRG1 is joined to the N-terminal of EGFP by the linker sequence AALELVDPPGSIAT. EGFP fluorescence and the immunofluorescence signal for the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, are colocalized in the late endocytic compartments of mutant peritoneal macrophages. Cre recombinase excision of the loxP-flanked DNA segment generates a null allele.
Colony maintenance兄妹交配特記 : ホモで継代可能。ホモは１年未満で繁殖成績低下。繁殖成績が低いその他半年以降、やや健康不良にみえる (毛並みが悪くなる)。その後、水頭症になる個体もある。
ReferencesJ. Cell Sci., 122, 2504-2513 (2009). 19549681

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tcirg1T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A319Tcirg1<tm1.1Ywd>targeted mutation 1.1, Yoh Wada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site19Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)19

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Ordering Information

供与核酸Phage phage P1 loxP sites, Yeast flipase recombination target site, Jellyfish GFP cDNA, Mouse Tcirg1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
公表を前提とした学術研究に限る。本件リソースの使用は共同研究のみに限定する。当該リソースを使用した研究成果の基づき特許等の申請を行う場合は、別途協議を行う。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途、MTA を締結する。
Depositor和田　洋（国立大学法人大阪大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data