Strain Data Sheet

RBRC04395

Strain Information

Image
BRC No.RBRC04395
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Siglec1<tm1(HBEGF)Mtka>
Former Common nameCD169-DTR KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development理化学研究所免疫・アレルギー科学総合研究所。R1 ES細胞を用いて作出。C57BL/6背景。
Strain descriptionCD169 (Siglec1) 遺伝子プロモーター下流にヒトジフテリア毒素受容体 (DTR) であるHB-EGF遺伝子を挿入したマウス。ジフテリア毒素 (DT) 投与により脾臓辺縁帯およびリンパ洞に局在するマクロファージ (CD169陽性マクロファージ) が消失する。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Critical role of macrophages in the marginal zone in the suppression of immune responses to apoptotic cell-associated antigens.
Miyake Y, Asano K, Kaise H, Uemura M, Nakayama M, Tanaka M
J. Clin. Invest., 117, 2268-2278 (2007). 17657313

Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice.
M Saito, T Iwawaki, C Taya, H Yonekawa, M Noda, Y Inui, E Mekada, Y Kimata, A Tsuru, K Kohno
Nat. Biotechnol., 19, 746-750 (2001). 11479567

Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice.
Saito M, Iwawaki T, Taya C, Yonekawa H, Noda M, Inui Y, Mekada E, Kimata Y, Tsuru A, Kohno K
Nat. Biotechnol., 19, 746-750 (2001). 11479567

Health Report

Examination Date / Room / Rack2024/06/10Room:3-2Rack:B
2024/03/11Room:3-2Rack:B
2023/12/11Room:3-2Rack:B
2023/09/11Room:3-2Rack:B
2023/06/13Room:3-2Rack:B
2023/03/13Room:3-2Rack:B
2022/12/12Room:3-2Rack:B

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBEGFheparin-binding EGF-like growth factor (human)2HBEGF
Siglec1sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1targeted mutation 1, Masato Tanaka
loxPphage P1 loxP2loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal dendritic cell physiology(MP:0002376)

  • abnormal immune system physiology(MP:0001790)

  • abnormal immune tolerance(MP:0005000)

  • abnormal macrophage physiology(MP:0002451)

  • abnormal metallophilic macrophage morphology(MP:0008241)
    • more 1 phenotypes
  • abnormal spleen marginal zone macrophage morphology(MP:0008240)
    • Detailed phenotype data

    Ordering Information

    供与核酸Human HB-EGF(DTR) cDNA, Phage P1 loxP site, Mouse Siglec1 genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
    研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Biotechnol., 19, 746-750 (2001). J. Clin Invest., 117, 2268-2278 (2007).
    研究成果の公表にあたっては、寄託者/開発者（Masato Tanaka, Ph.D.およびKenji Kohno, Ph.D.）へ謝辞の表明を必要とする。
    Depositor田中　正人（独立行政法人理化学研究所）
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    生体マウスを1~3ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Dec 2012

    BRC mice in Publications

    Chalot M, Barroca V, Devanand S, Hoffschir F, Romeo PH, Moreno SG.
    Deleterious effect of bone marrow-resident macrophages on hematopoietic stem cells in response to total body irradiation.
    Blood Adv 6(6) 1766-1779(2022) 35100346
    Borriello F, Poli V, Shrock E, Spreafico R, Liu X, Pishesha N, Carpenet C, Chou J, Di Gioia M, McGrath ME, Dillen CA, Barrett NA, Lacanfora L, Franco ME, Marongiu L, Iwakura Y, Pucci F, Kruppa MD, Ma Z, Lowman DW, Ensley HE, Nanishi E, Saito Y, O'Meara TR, Seo HS, Dhe-Paganon S, Dowling DJ, Frieman M, Elledge SJ, Levy O, Irvine DJ, Ploegh HL, Williams DL, Zanoni I.
    An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity.
    Cell 185(4) 614-629.e21(2022) 35148840
    Casella V, Domenjo-Vila E, Esteve-Codina A, Pedragosa M, Cebollada Rica P, Vidal E, de la Rubia I, López-Rodríguez C, Bocharov G, Argilaguet J, Meyerhans A.
    Differential kinetics of splenic CD169+ macrophage death is one underlying cause of virus infection fate regulation.
    Cell Death Dis 14(12) 838(2023) 38110339
    Oh DS, Oh JE, Jung HE, Lee HK.
    Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection.
    Front Immunol 8 819(2017) 28751894
    Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH.
    Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22.
    Inflammation 40(4) 1191-1203(2017) 28466432
    Jing W, Guo X, Wang G, Bi Y, Han L, Zhu Q, Qiu C, Tanaka M, Zhao Y.
    Breast cancer cells promote CD169+ macrophage-associated immunosuppression through JAK2-mediated PD-L1 upregulation on macrophages.
    Int Immunopharmacol 78 106012(2020) 31865052
    Jing W, Qin F, Guo X, Sun Y, Yan C, Qiu C, Tanaka M, Shi B, Zhao Y.
    G-CSF mediates lung injury in mice with adenine-induced acute kidney injury.
    Int Immunopharmacol 63 1-8(2018) 30056257
    Zhang J, Supakorndej T, Krambs JR, Rao M, Abou-Ezzi G, Ye RY, Li S, Trinkaus K, Link DC.
    Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking.
    J Clin Invest 129(7) 2920-2931(2019) 31039135
    Kim HJ, Park JH, Kim HC, Kim CW, Kang I, Lee HK.
    Blood monocyte-derived CD169+ macrophages contribute to antitumor immunity against glioblastoma.
    Nat Commun 13(1) 6211(2022) 36266311
    Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI.
    Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen.
    Proc Natl Acad Sci U S A 117(22) 12295-12305(2020) 32424104