Strain Data Sheet

RBRC04395

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC04395
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6;129-Siglec1<tm1(HBEGF)Mtka>
Former Common name	CD169-DTR KO
H-2 Haplotype
ES Cell line	R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development	理化学研究所免疫・アレルギー科学総合研究所。R1 ES細胞を用いて作出。C57BL/6背景。
Strain description	CD169 (Siglec1) 遺伝子プロモーター下流にヒトジフテリア毒素受容体 (DTR) であるHB-EGF遺伝子を挿入したマウス。ジフテリア毒素 (DT) 投与により脾臓辺縁帯およびリンパ洞に局在するマクロファージ (CD169陽性マクロファージ) が消失する。
Colony maintenance	Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
References	Nat. Biotechnol., 19, 746-750 (2001). 11479567 J. Clin. Invest., 117, 2268-2278 (2007). 17657313

Health Report
Examination Date / Room / Rack	2024/03/11Room:3-2Rack:B 2023/12/11Room:3-2Rack:B 2023/09/11Room:3-2Rack:B 2023/06/13Room:3-2Rack:B 2023/03/13Room:3-2Rack:B 2022/12/12Room:3-2Rack:B 2022/09/12Room:3-2Rack:B 2022/06/13Room:3-2Rack:B

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Siglec1sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(HBEGF)Mtka>targeted mutation 1, Masato Tanaka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBEGFheparin-binding EGF-like growth factor (human)2HBEGF Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP

Gene

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Siglec1sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(HBEGF)Mtka>targeted mutation 1, Masato Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

HBEGFheparin-binding EGF-like growth factor (human)2HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

loxPphage P1 loxP2loxP

Ordering Information
供与核酸	Human HB-EGF(DTR) cDNA, Phage P1 loxP site, Mouse Siglec1 genomic DNA
Research application	Cre/loxP system Immunology and Inflammation Research
提供条件	条件を付加する。利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Biotechnol., 19, 746-750 (2001). J. Clin Invest., 117, 2268-2278 (2007). 研究成果の公表にあたっては、寄託者/開発者（Masato Tanaka, Ph.D.およびKenji Kohno, Ph.D.）へ謝辞の表明を必要とする。
Depositor	田中　正人（独立行政法人理化学研究所）
Strain Status	生体凍結胚凍結精子
Strain Availability	凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能生体マウスを3~6ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR- Genetic Background Mouse of the Month Dec 2012

BRC mice in Publications

Chalot M, Barroca V, Devanand S, Hoffschir F, Romeo PH, Moreno SG. Deleterious effect of bone marrow-resident macrophages on hematopoietic stem cells in response to total body irradiation. Blood Adv 6(6) 1766-1779(2022) 35100346 Casella V, Domenjo-Vila E, Esteve-Codina A, Pedragosa M, Cebollada Rica P, Vidal E, de la Rubia I, López-Rodríguez C, Bocharov G, Argilaguet J, Meyerhans A. Differential kinetics of splenic CD169+ macrophage death is one underlying cause of virus infection fate regulation. Cell Death Dis 14(12) 838(2023) 38110339 Oh DS, Oh JE, Jung HE, Lee HK. Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection. Front Immunol 8 819(2017) 28751894 Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH. Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22. Inflammation 40(4) 1191-1203(2017) 28466432 Jing W, Guo X, Wang G, Bi Y, Han L, Zhu Q, Qiu C, Tanaka M, Zhao Y. Breast cancer cells promote CD169+ macrophage-associated immunosuppression through JAK2-mediated PD-L1 upregulation on macrophages. Int Immunopharmacol 78 106012(2020) 31865052 Jing W, Qin F, Guo X, Sun Y, Yan C, Qiu C, Tanaka M, Shi B, Zhao Y. G-CSF mediates lung injury in mice with adenine-induced acute kidney injury. Int Immunopharmacol 63 1-8(2018) 30056257 Zhang J, Supakorndej T, Krambs JR, Rao M, Abou-Ezzi G, Ye RY, Li S, Trinkaus K, Link DC. Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking. J Clin Invest 129(7) 2920-2931(2019) 31039135 Kim HJ, Park JH, Kim HC, Kim CW, Kang I, Lee HK. Blood monocyte-derived CD169+ macrophages contribute to antitumor immunity against glioblastoma. Nat Commun 13(1) 6211(2022) 36266311 Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI. Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen. Proc Natl Acad Sci U S A 117(22) 12295-12305(2020) 32424104

BRC mice in Publications

Chalot M, Barroca V, Devanand S, Hoffschir F, Romeo PH, Moreno SG.
Deleterious effect of bone marrow-resident macrophages on hematopoietic stem cells in response to total body irradiation.
Blood Adv 6(6) 1766-1779(2022) 35100346

Casella V, Domenjo-Vila E, Esteve-Codina A, Pedragosa M, Cebollada Rica P, Vidal E, de la Rubia I, López-Rodríguez C, Bocharov G, Argilaguet J, Meyerhans A.
Differential kinetics of splenic CD169+ macrophage death is one underlying cause of virus infection fate regulation.
Cell Death Dis 14(12) 838(2023) 38110339

Oh DS, Oh JE, Jung HE, Lee HK.
Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection.
Front Immunol 8 819(2017) 28751894

Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH.
Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22.
Inflammation 40(4) 1191-1203(2017) 28466432

Jing W, Guo X, Wang G, Bi Y, Han L, Zhu Q, Qiu C, Tanaka M, Zhao Y.
Breast cancer cells promote CD169+ macrophage-associated immunosuppression through JAK2-mediated PD-L1 upregulation on macrophages.
Int Immunopharmacol 78 106012(2020) 31865052

Jing W, Qin F, Guo X, Sun Y, Yan C, Qiu C, Tanaka M, Shi B, Zhao Y.
G-CSF mediates lung injury in mice with adenine-induced acute kidney injury.
Int Immunopharmacol 63 1-8(2018) 30056257

Zhang J, Supakorndej T, Krambs JR, Rao M, Abou-Ezzi G, Ye RY, Li S, Trinkaus K, Link DC.
Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking.
J Clin Invest 129(7) 2920-2931(2019) 31039135

Kim HJ, Park JH, Kim HC, Kim CW, Kang I, Lee HK.
Blood monocyte-derived CD169+ macrophages contribute to antitumor immunity against glioblastoma.
Nat Commun 13(1) 6211(2022) 36266311

Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI.
Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen.
Proc Natl Acad Sci U S A 117(22) 12295-12305(2020) 32424104

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications