Strain Data Sheet



BRC No.RBRC04395
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Siglec1<tm1(HBEGF)Mtka>
Former Common nameCD169-DTR KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain development理化学研究所免疫・アレルギー科学総合研究所。R1 ES細胞を用いて作出。C57BL/6背景。
Strain descriptionCD169 (Siglec1) 遺伝子プロモーター下流にヒトジフテリア毒素受容体 (DTR) であるHB-EGF遺伝子を挿入したマウス。ジフテリア毒素 (DT) 投与により脾臓辺縁帯およびリンパ洞に局在するマクロファージ (CD169陽性マクロファージ) が消失する。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNat. Biotechnol., 19, 746-750 (2001). 11479567
J. Clin. Invest., 117, 2268-2278 (2007). 17657313

Health Report

Examination Date / Room / Rack2023/03/13Room:3-2Rack:B


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Siglec1sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(HBEGF)Mtka>targeted mutation 1, Masato Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)2HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP


供与核酸Human HB-EGF(DTR) cDNA, Phage P1 loxP site, Mouse Siglec1 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Biotechnol., 19, 746-750 (2001). J. Clin Invest., 117, 2268-2278 (2007).
研究成果の公表にあたっては、寄託者/開発者(Masato Tanaka, Ph.D.およびKenji Kohno, Ph.D.)へ謝辞の表明を必要とする。
Depositor田中 正人(独立行政法人理化学研究所)
Strain Status生体のアイコン生体
Strain Availability凍結精子を1ヶ月以内に提供可能
Additional Info.Mouse of the Month Dec 2012
Genetic Background
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Chalot M, Barroca V, Devanand S, Hoffschir F, Roméo PH, Moreno SG.
Deleterious effect of bone marrow-resident macrophages on hematopoietic stem cells in response to total body irradiation.
Blood Adv (2022) 35100346

Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH.
Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22.
Inflammation 40(4) 1191-1203(2017) 28466432

Jing W, Guo X, Wang G, Bi Y, Han L, Zhu Q, Qiu C, Tanaka M, Zhao Y.
Breast cancer cells promote CD169+ macrophage-associated immunosuppression through JAK2-mediated PD-L1 upregulation on macrophages.
Int Immunopharmacol 78 106012(2020) 31865052

Jing W, Qin F, Guo X, Sun Y, Yan C, Qiu C, Tanaka M, Shi B, Zhao Y.
G-CSF mediates lung injury in mice with adenine-induced acute kidney injury.
Int Immunopharmacol 63 1-8(2018) 30056257

Zhang J, Supakorndej T, Krambs JR, Rao M, Abou-Ezzi G, Ye RY, Li S, Trinkaus K, Link DC.
Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking.
J Clin Invest 129(7) 2920-2931(2019) 31039135

Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI.
Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen.
Proc Natl Acad Sci U S A 117(22) 12295-12305(2020) 32424104