Information1 | |
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Image | |
BRC No. | RBRC04395 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129-Siglec1<tm1(HBEGF)Mtka> |
Former Common name | CD169-DTR KO |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | 理化学研究所免疫・アレルギー科学総合研究所。R1 ES細胞を用いて作出。C57BL/6背景。 |
Strain description | CD169 (Siglec1) 遺伝子プロモーター下流にヒトジフテリア毒素受容体 (DTR) であるHB-EGF遺伝子を挿入したマウス。ジフテリア毒素 (DT) 投与により脾臓辺縁帯およびリンパ洞に局在するマクロファージ (CD169陽性マクロファージ) が消失する。 |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Nat. Biotechnol., 19, 746-750 (2001). 11479567 J. Clin. Invest., 117, 2268-2278 (2007). 17657313 |
Health Report | |
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Examination Date / Room / Rack | 2023/03/13Room:3-2Rack:B 2022/12/12Room:3-2Rack:B 2022/09/12Room:3-2Rack:B 2022/06/13Room:3-2Rack:B 2022/03/14Room:3-2Rack:B 2021/12/14Room:3-2Rack:B 2021/10/11Room:3-2Rack:B 2021/10/11Room:3-2Rack:B 2021/09/13Room:3-2Rack:B 2021/06/14Room:3-2Rack:B |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Siglec1sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(HBEGF)Mtka>targeted mutation 1, Masato Tanaka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBEGFheparin-binding EGF-like growth factor (human)2HBEGF Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP |
Information2 | |
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供与核酸 | Human HB-EGF(DTR) cDNA, Phage P1 loxP site, Mouse Siglec1 genomic DNA |
Research application | Cre/loxP system Immunology and Inflammation Research |
提供条件 | 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。 研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Biotechnol., 19, 746-750 (2001). J. Clin Invest., 117, 2268-2278 (2007). 研究成果の公表にあたっては、寄託者/開発者(Masato Tanaka, Ph.D.およびKenji Kohno, Ph.D.)へ謝辞の表明を必要とする。 |
Depositor | 田中 正人(独立行政法人理化学研究所) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを3~6ヶ月以内に提供可能 |
Additional Info. | Mouse of the Month Dec 2012 Genetic Background Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Chalot M, Barroca V, Devanand S, Hoffschir F, Roméo PH, Moreno SG. Deleterious effect of bone marrow-resident macrophages on hematopoietic stem cells in response to total body irradiation. Blood Adv (2022) 35100346 Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH. Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22. Inflammation 40(4) 1191-1203(2017) 28466432 Jing W, Guo X, Wang G, Bi Y, Han L, Zhu Q, Qiu C, Tanaka M, Zhao Y. Breast cancer cells promote CD169+ macrophage-associated immunosuppression through JAK2-mediated PD-L1 upregulation on macrophages. Int Immunopharmacol 78 106012(2020) 31865052 Jing W, Qin F, Guo X, Sun Y, Yan C, Qiu C, Tanaka M, Shi B, Zhao Y. G-CSF mediates lung injury in mice with adenine-induced acute kidney injury. Int Immunopharmacol 63 1-8(2018) 30056257 Zhang J, Supakorndej T, Krambs JR, Rao M, Abou-Ezzi G, Ye RY, Li S, Trinkaus K, Link DC. Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking. J Clin Invest 129(7) 2920-2931(2019) 31039135 Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI. Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen. Proc Natl Acad Sci U S A 117(22) 12295-12305(2020) 32424104 |