Strain Data Sheet

RBRC04364

Strain Information

Image
BRC No.RBRC04364
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aqp4<tm1.1(GFP)Mysi>/5Rbrc
Former Common nameAquaporin-4 (AQP4) knockout mouse, AQP4 null mouse, aquaporin4 (GFP), aquaporin 4(GFP) (Acc.No. CDB0758K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development慶應義塾大学医学部・安井正人先生、理化学研究所発生・再生科学総合研究センター・動物資源開発室。
Strain descriptionaquaporin 4遺伝子のGFPノックインマウス。EGFP-Pgk-neo型 (#5変異ES由来) (B6混合背景：RBRC04397、BALB/c背景：RBRC04399) 、Pgk-neo除去型 (#5変異ES由来) (B6背景：RBRC04364、BALB/c混合背景：RBRC04400) 、EGFP-Pgk-neo除去型 (#5変異ES由来) (B6混合背景：RBRC04401、BALB/c混合背景：RBRC04402) 、EGFP-Pgk-neo型 (#50変異ES由来) (B6混合背景：RBRC04398) 、Pgk-neo除去型 (#50変異ES由来) (B6混合背景：RBRC04403) 。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesMol Cell Neurosci, 56, 65-75 (2013). 23428384
Proc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aqp4aquaporin 418Aqp4<tm1.1(GFP)>targeted mutation 1.1Mysi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)18EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Ordering Information

供与核酸phage P1 loxP sites, Jellyfish EGFP cDNA, yeast FRT (flipase recombination target) site, Mouse polyA signal, mouse Aqp4 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
(1) 当該マウスを用いる論文発表に際しては Accession No. CDB0758K-1、その WEB サイト（https://large.riken.jp/distribution/mutant-list.html）とともに当該マウスを記載すること。(2) 当該マウスを用いる論文発表に際しては、その由来として第一報論文（Mol Cell Neurosci, 56, 65-75 (2013). ）を引用すること。(3) 発表論文を寄託者(mutant.bdr@riken.jp)に連絡すること。
DepositorRIKEN CDB（独立行政法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Michael Nicosia, Juyeun Lee, Ashley Beavers, Danielle Kish, George W Farr, Paul R McGuirk, Marc F Pelletier, Justin D Lathia, Robert L Fairchild, Anna Valujskikh
Water channel Aquaporin 4 is required for T cell receptor mediated lymphocyte activation
Journal of Leukocyte Biology (2023) 36805947

Nicosia M, Miyairi S, Beavers A, Farr GW, McGuirk PR, Pelletier MF, Valujskikh A.
Aquaporin 4 inhibition alters chemokine receptor expression and T cell trafficking.
Sci Rep 9(1) 7417(2019) 31092872

Z Heřmanová
The Role of Aquaporin 4 channels and Transient Receptor Potential Vanilloid 4 channels in astrocytic swelling.
Univerzita Karlova (2019)