Strain Data Sheet

RBRC04335

Strain Information

Image
BRC No.RBRC04335
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3-Tg(Thy1-ChR2/mCherry)9-7Sasa/Rbrc
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by National Institute for Basic Biology and RIKEN BioResource Center in 2010.
Strain descriptionOptogenetic transgenic mice. A transgenic construct containing ChR2 (channelrhodopsin-2)-mCherry fusion cDNA under the control of the rat thymus cell antigen, theta promoter was microinjected into B6C3F1 fertilized eggs. Expression of ChR2/mCherry has not been examined yet. Users are encouraged to test the expression pattern and feed back the data for the research community.
Colony maintenanceCarrier x Noncarrier [C57BL/6NJcl]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)UNChR2rat Thy1 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Ordering Information

供与核酸mCherry [Discosoma sp.(class 1)], Thy1[rat genomic DNA(class 1)], COP4 (Channelrhodopsin-2)[CHLREDRAFT_182032, Chlamydomonas cDNA(class 1)],SV40 poly A [SV40 gene unit(class 2)]
Research application
提供条件条件を付加する。
研究成果の公表にあたっては、笹岡俊邦（基礎生物学研究所）と理研バイオリソースセンターに対して謝辞の表明を必要とする。研究成果の公表にあたって寄託者の指定する文献を引用する(後日指定)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
DepositorRIKEN BRC（独立行政法人理化学研究所）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data