Strain Data Sheet

RBRC04020

Strain Information

Image
BRC No.RBRC04020
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Dnase2a<tm2.1Osa>
Former Common nameDNaseII conditional knock out
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6
Appearance
Strain development
Strain description
Colony maintenance
References
Chronic polyarthritis caused by mammalian DNA that escapes from degradation in macrophages.
Kawane K, Ohtani M, Miwa K, Kizawa T, Kanbara Y, Yoshioka Y, Yoshikawa H, Nagata S
Nature, 443, 998-1002 (2006). 17066036

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dnase2adeoxyribonuclease II alpha8Dnase2atargeted mutation 2.1, Shigekazu Nagata
loxPphage P1 loxP8loxP
loxPphage P1 loxP8loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Dnase2a genomic DNA [update May 20, 2015]
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 443, 998-1002 (2006). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShigekazu Nagata (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Aug 2012

    BRC mice in Publications

    Chan MP, Onji M, Fukui R, Kawane K, Shibata T, Saitoh S, Ohto U, Shimizu T, Barber GN, Miyake K.
    DNase II-dependent DNA digestion is required for DNA sensing by TLR9.
    Nat Commun 6 5853(2015) 25600358