Strain Data Sheet

RBRC03733

Strain Information

Image
BRC No.RBRC03733
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnmt3b<tm5.1Enl>
Former Common nameDnmt3b<tm5.1Enl>; Dnmt3b conditional KO, Dnmt3b-2lox
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentMassachusetts General Hospital・岡野正樹先生、En Li（2001）。J1 ES細胞を用いて作出。
Strain descriptionDnmt3b遺伝子のfloxedマウス。Dnmt3b遺伝子座の酵素活性に必須なモチーフをコードするエクソンをloxP配列で挟んでおり、適切なCre発現マウスとの交配により、コンディショナルにDnmt3bを不活化することができる。哺乳類は、互いに高いアミノ酸配列類似性のある二つのDe novo型DNAメチル化酵素Dnmt3a、Dnmt3bを持つ。両者は異なるアイソフォーム、発現パターンを示し、互いに重複する機能とそれぞれに特異的な機能を持つ。ホモマウスは通常の維持繁殖には特に問題ない。Agouti遺伝子座に連鎖している。Dnmt3aノックアウトマウス (RBRC03730) 、Dnmt3a floxedマウス (RBRC03731) 、Dnmt3bノックアウトマウス (RBRC03732) 。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Biol. Chem., 280, 17986-17991 (2005). 15757890

Health Report

Examination Date / Room / Rack2022/06/13Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnmt3bDNA methyltransferase 3B2Dnmt3b<tm5.1Enl>targeted mutation 5.1, En Li

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

供与核酸Phage P1 LoxP sites, mouse Dnmt3b genomic DNA
Research applicationCre/loxP system
提供条件条件を付加する。
A. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature J Biol Chem. 2005 280:17986-91 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient.
Depositor岡野　正樹（独立行政法人理化学研究所とMGH）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Please use the MATERIAL TRANSFER AGREEMENT for Distribution for non-profit or for profit (English only).
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Matsuzaki H, Okamura E, Takahashi T, Ushiki A, Nakamura T, Nakano T, Hata K, Fukamizu A, Tanimoto K.
De novo DNA methylation through the 5'-segment of the H19 ICR maintains its imprint during early embryogenesis.
Development 142(22) 3833-44(2015) 26417043