Strain Information | |
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Image | |
BRC No. | RBRC03731 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S4-Dnmt3a<tm3.1Enl> |
Former Common name | Dnmt3a<tm3.1Enl>; Dnmt3a conditional KO, Dnmt3a-2lox |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Massachusetts General Hospital・岡野正樹先生、En Li(2001)。J1 ES細胞を用いて作出。 |
Strain description | Dnmt3a遺伝子のfloxedマウス。Dnmt3a遺伝子座の酵素活性に必須なモチーフをコードするエクソンをloxP配列で挟んでおり、適切なCre発現マウスとの交配により、コンディショナルにDnmt3aを不活化することができる。哺乳類は、互いに高いアミノ酸配列類似性のある二つのDe novo型DNAメチル化酵素Dnmt3a、Dnmt3bを持つ。両者は異なるアイソフォーム、発現パターンを示し、互いに重複する機能とそれぞれに特異的な機能を持つ。ホモマウスは通常の維持繁殖には特に問題ない。TNAP-Creトランスジェニックマウスと交配し、生殖細胞特異的にDnmt3aを不活性化すると、ゲノムインプリントが形成されない。Dnmt3aノックアウトマウス (RBRC03730) 、Dnmt3bノックアウトマウス (RBRC03732) 、Dnmt3b floxedマウス (RBRC03733) 。 |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Nature, 429, 900-903 (2004). 15215868 |
Health Report | |
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Examination Date / Room / Rack | 2024/03/11Room:3-2Rack:B 2023/12/11Room:3-2Rack:B 2023/09/11Room:3-2Rack:B 2023/06/13Room:3-2Rack:B 2023/03/13Room:3-2Rack:B 2022/12/12Room:3-2Rack:B 2022/09/12Room:3-2Rack:B 2022/06/13Room:3-2Rack:B |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP |
Ordering Information | |
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供与核酸 | Phage P1 LoxP sites, mouse Dnmt3a genomic DNA |
Research application | Cre/loxP system |
提供条件 | 条件を付加する。 A. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature Nature 2004 429:900-3 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient. |
Depositor | 岡野 正樹(独立行政法人理化学研究所とMGH) |
Strain Status | 生体 凍結胚 凍結精子 |
Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを1~3ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Hatazawa Y, Ono Y, Hirose Y, Kanai S, Fujii NL, Machida S, Nishino I, Shimizu T, Okano M, Kamei Y, Ogawa Y. Reduced Dnmt3a increases Gdf5 expression with suppressed satellite cell differentiation and impaired skeletal muscle regeneration. FASEB J 32(3) 1452-1467(2018) 29146735 Sharma A, Klein SS, Barboza L, Lohdi N, Toth M. Principles Governing DNA Methylation during Neuronal Lineage and Subtype Specification. J Neurosci 36(5) 1711-22(2016) 26843651 |