Strain Data Sheet

RBRC03708

Strain Information

Image
BRC No.RBRC03708
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameFLS.B6-Lep<ob>/Shi
Former Common nameFLS-ob
H-2 Haplotype
ES Cell line
Background strainFLS
Appearance
Strain developmentDeveloped by Aburahi Laboratories, Shionogi Research Laboratories, Shionogi & Co., Ltd. in 1990.
Strain description
Colony maintenance
References
Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis.
T Hirasawa, T Ohara, S Makino
Exp. Anim., 46, 75-78 (1997). 9027475

Mol. Med., 36, 448-451 (1999).

Characterization of the effects of pancreatic polypeptide in the regulation of energy balance.
Akihiro Asakawa, Akio Inui, Hideki Yuzuriha, Naohiko Ueno, Goro Katsuura, Mineko Fujimiya, Masayuki A Fujino, Akira Niijima, Michael M Meguid, Masato Kasuga
Gastroenterology., 124, 1325-1336 (2003). 12730873

Health Report

Examination Date / Room / Rack2025/10/20Room:4-BRack:JSentinel mouse program
2025/08/04Room:4-BRack:JSentinel mouse program
2025/05/26Room:4-BRack:JSentinel mouse program
2025/02/25Room:4-BRack:JSentinel mouse program
2024/11/25Room:4-BRack:JSentinel mouse program
2024/08/26Room:4-BRack:JSentinel mouse program
2024/05/27Room:4-BRack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Lep
MGI:104663		leptin6Lep<ob>
MGI:1856424		obese
  • obesity due to congenital leptin deficiency(DisGeNET, MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal acute inflammation (MP:0002498)

  • abnormal adipose tissue amount (MP:0005452)

  • abnormal aerobic respiration (MP:0010957)

  • abnormal arteriole morphology (MP:0004112)

  • abnormal behavior (MP:0004924)
    • more 174 phenotypes
  • abnormal body weight (MP:0001259)

  • abnormal bone marrow morphology (MP:0002397)

  • abnormal branching of the mammary ductal tree (MP:0000662)

  • abnormal cardiovascular system physiology (MP:0001544)

  • abnormal cell cycle (MP:0003077)

  • abnormal chemokine secretion (MP:0008722)

  • abnormal circulating cholesterol level (MP:0000180)

  • abnormal circulating glucose level (MP:0000188)

  • abnormal circulating hormone level (MP:0005418)

  • abnormal circulating pancreatic peptide level (MP:0008459)

  • abnormal colon goblet cell morphology (MP:0013795)

  • abnormal cortical plate morphology (MP:0008439)

  • abnormal corticosterone level (MP:0003963)

  • abnormal digestive system physiology (MP:0001663)

  • abnormal drinking behavior (MP:0001422)

  • abnormal eating behavior (MP:0001431)

  • abnormal embryonic neuroepithelium morphology (MP:0004261)

  • abnormal epidermal layer morphology (MP:0001216)

  • abnormal epididymal fat pad morphology (MP:0006319)

  • abnormal fat cell morphology (MP:0009115)

  • abnormal food intake (MP:0005449)

  • abnormal frequency of paradoxical sleep (MP:0003194)

  • abnormal gland physiology (MP:0002164)

  • abnormal glucose homeostasis (MP:0002078)

  • abnormal hepatocyte physiology (MP:0010080)

  • abnormal hormone level (MP:0003953)

  • abnormal insulin secretion (MP:0003564)

  • abnormal intestinal epithelium morphology (MP:0000488)

  • abnormal intestinal mucosa morphology (MP:0000511)

  • abnormal intestine morphology (MP:0000477)

  • abnormal lipid level (MP:0001547)

  • abnormal liver weight (MP:0004847)

  • abnormal long bone epiphyseal plate morphology (MP:0003055)

  • abnormal long bone epiphyseal plate proliferative zone (MP:0003662)

  • abnormal long bone hypertrophic chondrocyte zone (MP:0000165)

  • abnormal microglial cell physiology (MP:0003691)

  • abnormal muscle contractility (MP:0005620)

  • abnormal muscle morphology (MP:0002108)

  • abnormal myocardial fiber morphology (MP:0000278)

  • abnormal myocardial fiber physiology (MP:0004215)

  • abnormal osteoclast differentiation (MP:0008396)

  • abnormal ovarian folliculogenesis (MP:0001130)

  • abnormal oxygen consumption (MP:0005288)

  • abnormal pain threshold (MP:0001970)

  • abnormal pancreatic beta cell morphology (MP:0005217)

  • abnormal pancreatic islet morphology (MP:0005215)

  • abnormal podocyte morphology (MP:0005326)

  • abnormal postnatal growth (MP:0001731)

  • abnormal protein level (MP:0008469)

  • abnormal respiratory electron transport chain (MP:0010955)

  • abnormal response to cardiac infarction (MP:0000343)

  • abnormal retina vasculature morphology (MP:0002792)

  • abnormal skeletal muscle fiber type ratio (MP:0009409)

  • abnormal skeleton development (MP:0002113)

  • abnormal sleep pattern (MP:0001501)

  • abnormal sympathetic nervous system physiology (MP:0004183)

  • abnormal urine catecholamine level (MP:0011478)

  • abnormal urine homeostasis (MP:0009643)

  • abnormal vagina orifice morphology (MP:0001142)

  • abnormal vascular wound healing (MP:0004883)

  • absent corpus luteum (MP:0001134)

  • albuminuria (MP:0002871)

  • allodynia (MP:0003177)

  • cardiovascular system phenotype (MP:0005385)

  • decreased CD4-positive, alpha-beta T cell number (MP:0008075)

  • decreased NK cell number (MP:0008045)

  • decreased T cell proliferation (MP:0005095)

  • decreased activity of thyroid gland (MP:0003503)

  • decreased body length (MP:0001258)

  • decreased body temperature (MP:0005534)

  • decreased bone mass (MP:0004016)

  • decreased cardiac muscle contractility (MP:0005140)

  • decreased circulating glucose level (MP:0005560)

  • decreased circulating testosterone level (MP:0002780)

  • decreased circulating tumor necrosis factor level (MP:0008554)

  • decreased embryonic neuroepithelial cell proliferation (MP:0012706)

  • decreased embryonic neuroepithelium thickness (MP:0012703)

  • decreased food intake (MP:0011940)

  • decreased insulin secretion (MP:0003059)

  • decreased lean body mass (MP:0003961)

  • decreased length of long bones (MP:0004686)

  • decreased liver triglyceride level (MP:0009356)

  • decreased locomotor activity (MP:0001402)

  • decreased nerve conduction velocity (MP:0008814)

  • decreased oxygen consumption (MP:0005290)

  • decreased pancreatic beta cell mass (MP:0009114)

  • decreased pancreatic beta cell number (MP:0003339)

  • decreased seminal vesicle weight (MP:0004910)

  • decreased skeletal muscle fiber size (MP:0009400)

  • decreased skeletal muscle mass (MP:0004819)

  • decreased splenocyte number (MP:0009339)

  • decreased susceptibility to hepatic steatosis (MP:0002310)

  • decreased testis weight (MP:0004852)

  • decreased white fat cell number (MP:0009131)

  • disorganized pancreatic islets (MP:0009254)

  • enlarged kidney (MP:0003068)

  • enlarged pancreatic islets (MP:0009171)

  • expanded mesangial matrix (MP:0011353)

  • female infertility (MP:0001926)

  • fragmentation of sleep/wake states (MP:0009436)

  • glomerulosclerosis (MP:0005264)

  • heart left ventricle hypertrophy (MP:0002625)

  • hepatic steatosis (MP:0002628)

  • hyperalgesia (MP:0005407)

  • hyperglycemia (MP:0001559)

  • hypersecretion of corticosterone (MP:0001744)

  • hypoglycemia (MP:0000189)

  • hypotension (MP:0001596)

  • impaired adaptive thermogenesis (MP:0011049)

  • impaired glucose tolerance (MP:0005293)

  • increased adrenocorticotropin level (MP:0005129)

  • increased blood urea nitrogen level (MP:0005565)

  • increased body length (MP:0001257)

  • increased body weight (MP:0001260)

  • increased bone mass (MP:0005605)

  • increased cholesterol level (MP:0003982)

  • increased circulating HDL cholesterol level (MP:0001556)

  • increased circulating LDL cholesterol level (MP:0000182)

  • increased circulating VLDL cholesterol level (MP:0005145)

  • increased circulating cholesterol level (MP:0005178)

  • increased circulating corticosterone level (MP:0001745)

  • increased circulating insulin level (MP:0002079)

  • increased circulating leptin level (MP:0005669)

  • increased circulating triglyceride level (MP:0001552)

  • increased fasting circulating glucose level (MP:0013279)

  • increased fat cell size (MP:0006094)

  • increased food intake (MP:0011939)

  • increased gonadal fat pad weight (MP:0009285)

  • increased growth rate (MP:0002865)

  • increased heart weight (MP:0002833)

  • increased inguinal fat pad weight (MP:0009292)

  • increased insulin secretion (MP:0003058)

  • increased interleukin-6 secretion (MP:0008705)

  • increased interscapular fat pad weight (MP:0009294)

  • increased kidney weight (MP:0003917)

  • increased lean body mass (MP:0003960)

  • increased liver cholesterol level (MP:0010027)

  • increased liver triglyceride level (MP:0009355)

  • increased liver weight (MP:0002981)

  • increased metastatic potential (MP:0001272)

  • increased myocardial fiber size (MP:0004564)

  • increased oxygen consumption (MP:0005289)

  • increased pancreas weight (MP:0009108)

  • increased pancreatic beta cell mass (MP:0009113)

  • increased pancreatic beta cell number (MP:0003645)

  • increased pancreatic islet number (MP:0009167)

  • increased percent body fat/body weight (MP:0005458)

  • increased renal glomerulus basement membrane thickness (MP:0011409)

  • increased susceptibility to endotoxin shock (MP:0008735)

  • increased thermal nociceptive threshold (MP:0001973)

  • increased urine glucose level (MP:0001759)

  • infertility (MP:0001924)

  • insulin resistance (MP:0005331)

  • liver inflammation (MP:0001860)

  • lung inflammation (MP:0001861)

  • male infertility (MP:0001925)

  • mesangiolysis (MP:0011430)

  • obese (MP:0001261)

  • osteopetrosis (MP:0000067)

  • oxidative stress (MP:0003674)

  • pancreatic islet hyperplasia (MP:0005491)

  • polyphagia (MP:0001433)

  • polyuria (MP:0001762)

  • postnatal lethality, incomplete penetrance (MP:0011086)

  • premature death (MP:0002083)

  • prolonged diestrus (MP:0009011)

  • prolonged estrous cycle (MP:0009006)

  • prolonged estrus (MP:0009017)

  • reduced male fertility (MP:0001922)

  • renal glomerulus hypertrophy (MP:0011294)

  • renal interstitial fibrosis (MP:0003215)

  • short femur (MP:0003109)

  • short humerus (MP:0004351)

  • small pancreatic islets (MP:0009172)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationCancer Research
    Metabolism Research
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must inform the DEPOSITOR (srp-contact@shionogi.co.jp) the research plan using the BIOLOGICAL RESOURCE and must obtain a prior written consent from the DEPOSITOR using the APPROVAL FROM specified by the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must inform the DEPOSITOR of the contents of such publication in advance. The RECIPIENT must contact and discuss with the DEPOSITOR in case of filing a patent application based on research results by using the BIOLOGICAL RESOURCES. The RECIPIENT must contact and discuss with the DEPOSITOR in case of application for any commercial use.
    DepositorShionogi & Co., Ltd. (Shionogi & Co., Ltd)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
      4. Please contact "srp-contact@shionogi.co.jp" to obtain the specific application form for the research plan.

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data