Strain Data Sheet

RBRC03705

Strain Information

Image
BRC No.RBRC03705
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(Nes-cre)52Utr/Rbrc
Former Common nameNestin-NLSCre
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Laboratory Animal Resource Center, Tsukuba University and RIKEN BioResource Center in 2009. C57BL/6N background.
Strain descriptionCre-driver transgenic mice. A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse nestin promoter fragment derived from the BAC library (BAC clone RP24-64E17)was microinjected into C57BL/6NCrlCrlj fertilized eggs. Tissue-specific expression of Cre recombinase has not been examined yet. Users are encouraged to test the tissue-specificity and feed back the data for the research community.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnlsmouse Nestin promoter (neuronal-specific promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Ordering Information

供与核酸Cre recombinase [P1_ap003, Bacteriophage genomic DNA (class 1)], FRT sequence [yeast genomic DNA (class 1)], Nestin [mouse genomic DNA (neuronal-specific promoter)(class 1)], Phage P1 loxP derived from pBACe3.6 vector, BAC clone RP23-141A13, SV40 poly A, SV40 Large T antigen nuclear localization signal (NLS)
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。
研究成果の公表にあたっては、八神健一（筑波大学生命科学動物資源センター）と理研バイオリソースセンターに対して謝辞の表明を必要とする。研究成果の公表にあたって寄託者の指定する文献を引用する(後日指定)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
DepositorRIKEN BRC（独立行政法人理化学研究所）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data