Strain Data Sheet

RBRC03519

Strain Information

Image
BRC No.RBRC03519
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2;129P2-Cthrc1<tm1Hssk>/Rbrc
Former Common nameCthrc1(lacZ) (Acc.No. CDB0502K)
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Sasaki, RIKEN Center for Developmental Biology. A lacZ and PGK-neo cassette was transferred into E14TG2a ES cells to replace the exon 1 of Cthrc1 gene.
Strain descriptionCthrc1 gene knockout mice. Cthrc1 is a Wnt signaling component that selectively activates the PCP pathway.
Colony maintenance
ReferencesDev. Cell., 15, 23-36 (2008). 18606138

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cthrc1collagen triple helix repeat containing 115Cthrc1<tm1Hssk>targeted mutation 1, Hiroshi Ssasaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin 3' end15HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence15lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15

Ordering Information

供与核酸Neo : E. coli, lacZ : E. coli, lox71 and loxP : P1 Phage, frt : yeast FRT (flipase recombination target) sites, PGK promoter and PGK 3' end : mouse, rabbit beta-globin 3' end : Rabbit
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
(1) 当該マウスを用いる論文発表に際してはAccession No. CDB0502K、そのWEB サイトhttps://large.riken.jp/distribution/mutant-list.htmlとともに当該マウスを記載すること。(2) 当該マウスを用いる論文発表に際しては、その由来として第一報論文 Dev Cell. 2008 15(1):23-36. を引用すること。(3) 発表論文を寄託者（mutant.bdr@riken.jp）に連絡すること。
DepositorRIKEN CDB（独立行政法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Li J, Wang Y, Ma M, Jiang S, Zhang X, Zhang Y, Yang X, Xu C, Tian G, Li Q, Wang Y, Zhu L, Nie H, Feng M, Xia Q, Gu J, Xu Q, Zhang Z.
Autocrine CTHRC1 activates hepatic stellate cells and promotes liver fibrosis by activating TGF-β signaling.
EBioMedicine 40 43-55(2019) 30639416