Strain Data Sheet

RBRC03444

Strain Information

Image
BRC No.RBRC03444
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Pitx2<tm1Kki>/Rbrc
Former Common namePitx2 KO
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Mouse Pitx2 deficiency leads to anomalies of the ventral body wall, heart, extra- and periocular mesoderm and right pulmonary isomerism.
Kitamura K, Miura H, Miyagawa-Tomita S, Yanazawa M, Katoh-Fukui Y, Suzuki R, Ohuchi H, Suehiro A, Motegi Y, Nakahara Y, Kondo S, Yokoyama M
Development, 126(24) 5749-5758 (1999). 10572050

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ires internal ribosomal entry site (EMCV)3
Pitx2
MGI:109340		paired-like homeodomain transcription factor 23Pitx2<tm1Kki>
MGI:1857880		targeted mutation 1, Kunio Kitamura
  • anterior segment dysgenesis 4(MedGEN)

  • Axenfeld-Rieger syndrome type 1(DisGeNET, MedGEN)

  • ring dermoid of cornea(MedGEN)
    • lacZ beta-galactosidase (E. coli)3
    neo neomycin resistance gene (E. coli)3 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal atrioventricular cushion morphology(MP:0000297)

  • abnormal cornea morphology(MP:0001312)

  • abnormal digestive system development(MP:0003119)

  • abnormal extraocular muscle morphology(MP:0005247)

  • abnormal fetal atrioventricular canal morphology(MP:0006107)
    • more 16 phenotypes
  • abnormal heart and great artery attachment(MP:0010426)

  • abnormal heart development(MP:0000267)

  • abnormal mesoderm development(MP:0001680)

  • abnormal rib development(MP:0002823)

  • abnormal thoracic cage morphology(MP:0004624)

  • abnormal ventral body wall morphology(MP:0003384)

  • absent extraocular muscles(MP:0030171)

  • common atrioventricular valve(MP:0010607)

  • common atrium(MP:0010406)

  • double outlet right ventricle(MP:0000284)

  • enophthalmos(MP:0006198)

  • heart right ventricle hypoplasia(MP:0010422)

  • increased heart left atrium size(MP:0008726)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • right pulmonary isomerism(MP:0000531)

  • splayed ribs(MP:0004673)
    • Detailed phenotype data

    Ordering Information

    Donor DNAncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli beta-galactosidase, MC1 promoter (herpes simplex virus thymidine kinase promoter (HSV tk promoter)), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Pitx2genomic DNA
    Research application
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
    The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
    In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
    The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
    DepositorKunio Kitamura (Mitsubishi Kasei Institute of Life Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data