Strain Data Sheet

RBRC03418

Strain Information

Image
BRC No.RBRC03418
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Apoe<tm(APOE4)Sfu>/SfuRbrc
Former Common nameApoE4 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shinobu Fujita, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were wsed. The mice were crossed to C57BL/6.
Strain descriptionHuman ApoE4 knock-in mice. The mouse ApoE sequences including part of exon 2, entire exon 3 and most of exon 4 was replaced by the human APOE4 and a floxed neo casette.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NJcl]
ReferencesHum. Mol. Genet., 9(3):353-361 (2000). 10655544

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Apoeapolipoprotein E7Apoe<tm>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
APOE4apolipoprotein E4 (human)7APOE4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAhuman APOE4 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Apoe genomic DNA
Research applicationCardiovascular Research
Cre/loxP system
Hematological Research
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
DepositorShinobu Fujita (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Takebayashi Y, Yamazaki Y, Yamada H, Yazawa K, Nakamori M, Kurashige T, Morino H, Takahashi T, Sotomaru Y, Maruyama H.
Apolipoprotein E genotype-dependent accumulation of amyloid β in APP-knock-in mouse model of Alzheimer's disease.
Biochem Biophys Res Commun 683 149106(2023) 37857162

Yoshiko Takebayashi, Yu Yamazaki, Hidetada Yamada, Kyosuke Yazawa, Masahiro Nakamori, Takashi Kurashige, Hiroyuki Morino, Tetsuya Takahashi, Yusuke Sotomaru, Hirofumi Maruyama
Apolipoprotein E genotype-dependent accumulation of amyloid β in APP-knock-in mouse model of Alzheimer's disease
Biochemical and Biophysical Research Communications (2023)

Honda K, Saito Y, Saito H, Toyoda M, Abe R, Saito T, Saido TC, Michikawa M, Taru H, Sobu Y, Hata S, Nakaya T, Suzuki T.
Accumulation of amyloid-β in the brain of mouse models of Alzheimer's disease is modified by altered gene expression in the presence of human apoE isoforms during aging.
Neurobiol Aging 123 63-74(2023) 36638682