Strain Data Sheet

RBRC03390

Strain Information

Image
BRC No.RBRC03390
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Apoe<tm>/3SfuRbrc
Former Common nameApoE3-3 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development作出系統：129, C57BL/6N表現型：マウスアポEの替わりにヒトアポE3を発現三菱化学生命科学研究所 藤田 忍先生。
Strain descriptionマウスApoe遺伝子のエクソン2の19bp以降をヒトApoE3 cDNAおよびfloxed neo耐性遺伝子で置換したノックインマウス。マウスApoEの代わりにヒトApoE3を発現する。ライン3。
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References
Altered cholesterol metabolism in human apolipoprotein E4 knock-in mice.
H Hamanaka, Y Katoh-Fukui, K Suzuki, M Kobayashi, R Suzuki, Y Motegi, Y Nakahara, A Takeshita, M Kawai, K Ishiguro, M Yokoyama, S C Fujita
Hum. Mol. Genet., 9(3):353-361 (2000). 10655544

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
APOE3 apolipoprotein E3 (human)7APOE3
Apoe
MGI:88057		apolipoprotein E7Apoe
  • age related macular degeneration 1(MedGEN)

  • Alzheimer disease 2(DisGeNET, MedGEN)

  • Alzheimer disease 3(MedGEN)
    • more 4 Diseases
  • Alzheimer disease 4(MedGEN)

  • hyperlipoproteinemia type 3(DisGeNET, MedGEN)

  • lipoprotein glomerulopathy(DisGeNET, MedGEN)

  • sea-blue histiocyte syndrome(MedGEN)
    • loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP
    neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal brain morphology (MP:0002152)

  • abnormal circulating cholesterol level (MP:0000180)

  • abnormal phospholipid level (MP:0004777)

  • abnormal plasma membrane morphology (MP:0003950)

  • abnormal response to CNS ischemic injury (MP:0003075)
    • more 4 phenotypes
  • decreased brain cholesterol level (MP:0010161)

  • increased circulating LDL cholesterol level (MP:0000182)

  • increased circulating VLDL cholesterol level (MP:0005145)

  • increased circulating cholesterol level (MP:0005178)
    • Detailed phenotype data

    Ordering Information

    供与核酸human APOE3 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Apoe genomic DNA
    Research applicationCardiovascular Research
    Cre/loxP system
    Hematological Research
    提供条件1. 本件リソースの利用は非営利機関による学術研究に限る。
    2. 本件リソースを非営利機関による学術研究以外の目的で使用する場合には、事前に理研BRCに連絡し、三菱化学(株)と協議する。
    3. 本件リソースを用いた研究結果を論文投稿、学会発表等により公表する場合には、その由来を明記し、三菱化学(株)に謝意を表明する。
    4. 理研BRCは提供先機関名、研究責任者名、利用課題名および提供日を三菱化学(株)に年1回報告する。
    Depositor藤田　忍（(株)三菱化学生命科学研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Takebayashi Y, Yamazaki Y, Yamada H, Yazawa K, Nakamori M, Kurashige T, Morino H, Takahashi T, Sotomaru Y, Maruyama H.
    Apolipoprotein E genotype-dependent accumulation of amyloid β in APP-knock-in mouse model of Alzheimer's disease.
    Biochem Biophys Res Commun 683 149106(2023) 37857162
    Mok KK, Yeung SH, Cheng GW, Ma IW, Lee RH, Herrup K, Tse KH.
    Apolipoprotein E ε4 disrupts oligodendrocyte differentiation by interfering with astrocyte-derived lipid transport.
    J Neurochem 165(1) 55-75(2023) 36549843
    Honda K, Saito Y, Saito H, Toyoda M, Abe R, Saito T, Saido TC, Michikawa M, Taru H, Sobu Y, Hata S, Nakaya T, Suzuki T.
    Accumulation of amyloid-β in the brain of mouse models of Alzheimer's disease is modified by altered gene expression in the presence of human apoE isoforms during aging.
    Neurobiol Aging 123 63-74(2023) 36638682
    Cheng GW, Mok KK, Yeung SH, Kofler J, Herrup K, Tse KH.
    Apolipoprotein E ε4 Mediates Myelin Breakdown by Targeting Oligodendrocytes in Sporadic Alzheimer Disease.
    J Neuropathol Exp Neurol 81(9) 717-730(2022) 35779013