Strain Data Sheet

RBRC03241

Strain Information

Image
BRC No.RBRC03241
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(TCRaTCRb) Rag2<tm1Fwa>/Rbrc
Former Common nameBOG8 Tg, B6.Cg-Tg(TCRaTCRb) Rag2<tm1>/Rbrc
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, et a l
Cell, 68 (5) 855-67 (1992). 1547487

In vitro differentiation and commitment of CD4+ CD8+ thymocytes to the CD4 lineage, without TCR engagement.
Ohoka Y, Kuwata T, Tozawa Y, Zhao Y, Mukai M, Motegi Y, Suzuki R, Yokoyama M, Iwata M
Int. Immunol., 8(3): 297-306 (1996). 8671615

Differential induction of helper and killer T cells from isolated CD4+CD8+ thymocytes in suspension culture.
Iwata M, Kuwata T, Mukai M, Tozawa Y, Yokoyama M
Eur. J. Immunol., 26(9): 2081-2086 (1996). 8814250

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rag2
MGI:97849		recombination activating gene 22Rag2<tm1Fwa>
MGI:1858556		targeted mutation 1, Frederik W Alt
  • combined immunodeficiency with skin granulomas(MedGEN)

  • Omenn syndrome(DisGeNET, MedGEN)

  • severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-positive(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)2 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Tcra T cell receptor alpha chainUNTcra
    Tcrb T cell receptor beta chainUNTcrb

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMMLVプロモーターを上流に持つマウスTCRAVJ48C1遺伝子のcDNAとマウスTCRBV8.2DJ1.1C2を含むマウスTCRbeta鎖のgenomic DNAを挿入した。RAG-2遺伝子のexonをNeo遺伝子に置換
    Research application
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
    The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
    In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
    The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
    DepositorMakoto Iwata (Mitsubishi Kasei Institute of Life Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Dr Iwata Lab (English)

    BRC mice in Publications

    No Data