Strain Data Sheet
RBRC03163
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC03163 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6J.129P2-Hmox1<tm1Mym> |
| Former Common name | HO-1 floxed mouse |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | C57BL/6JJmsSlc |
| Appearance | |
| Strain development | 筑波大学先端学際領域研究センター・山本雅之先生（2001）。C57BL/6背景。 |
| Strain description | Hmox1 (Heme oxygenase-1) 遺伝子のfloxedマウス。エクソン3から5がloxPで挟まれている。組織臓器特異的Creマウスとの交配によりコンディショナルノックアウトマウスが作製可能。Hmox1遺伝子はヘムを分解し、ビリベルジン、一酸化炭素、鉄を生成する反応を触媒する酵素であるHeme oxygenase (HO) のisyozymeの一つ。 |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] |
| References | Tohoku J. Exp. Med., 216, 331-339 (2008). 19060448 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/01/22Room:3-CRack:C 2023/10/23Room:3-CRack:C 2023/07/24Room:3-CRack:C 2023/04/24Room:3-CRack:C 2023/01/23Room:3-CRack:C 2022/10/25Room:3-CRack:C 2022/08/22Room:3-CRack:C 2022/07/25Room:3-CRack:C |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter DsRed2Red Fluorescent Protein (Discosoma sp.)8DsRed2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP |
Ordering Information | |
|---|---|
| 供与核酸 | Bacteriophage loxP sites, chimeric intron and splicing acceptor (from pRL-null), Discosoma sp. DsRed2 cDNA, SV40 polyA signal, mouse Heme oxygenase-1 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| 提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。Tohoku J. Exp. Med., 216, 331-339 (2008). 研究成果の公表にあたって謝辞の表明を必要とする。 寄託後2年間は共著を必要とする共同研究とする。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は寄託者と別途協議を行う。 |
| Depositor | 山本 雅之（国立大学法人東北大学） |
| Strain Status |  生体 凍結胚 凍結精子 |
| Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを1~3ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Genetic Background |
BRC mice in Publications |
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Parada E, Egea J, Buendia I, Negredo P, Cunha AC, Cardoso S, Soares MP, López MG. The microglial α7-acetylcholine nicotinic receptor is a key element in promoting neuroprotection by inducing heme oxygenase-1 via nuclear factor erythroid-2-related factor 2. Antioxid Redox Signal 19(11) 1135-48(2013) 23311871 Alves de Souza RW, Gallo D, Lee GR, Katsuyama E, Schaufler A, Weber J, Csizmadia E, Tsokos GC, Koch LG, Britton SL, Wisløff U, Brum PC, Otterbein LE. Skeletal muscle heme oxygenase-1 activity regulates aerobic capacity. Cell Rep 35(3) 109018(2021) 33882313 Di Pietro C, Öz HH, Zhang PX, Cheng EC, Martis V, Bonfield TL, Kelley TJ, Jubin R, Abuchowski A, Krause DS, Egan ME, Murray TS, Bruscia EM. Recruitment of monocytes primed to express heme oxygenase-1 ameliorates pathological lung inflammation in cystic fibrosis. Exp Mol Med 54(5) 639-652(2022) 35581352 Schallner N, Pandit R, LeBlanc R 3rd, Thomas AJ, Ogilvy CS, Zuckerbraun BS, Gallo D, Otterbein LE, Hanafy KA. Microglia regulate blood clearance in subarachnoid hemorrhage by heme oxygenase-1. J Clin Invest 125(7) 2609-25(2015) 26011640 Parada E, Buendia I, Navarro E, Avendaño C, Egea J, López MG. Microglial HO-1 induction by curcumin provides antioxidant, antineuroinflammatory, and glioprotective effects. Mol Nutr Food Res 59(9) 1690-700(2015) 26047311 Fernández-Mendívil C, Luengo E, Trigo-Alonso P, García-Magro N, Negredo P, López MG. Protective role of microglial HO-1 blockade in aging: Implication of iron metabolism. Redox Biol 38 101789(2021) 33212416 |