Strain Data Sheet

RBRC02928

Strain Information

Image
BRC No.RBRC02928
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Olfr1507<tm1(EGFP)Hsak>
Former Common nameMOR28 knock-in
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development開発者／機関：石井智宏先生、坂野仁先生開発年：2000年
Strain descriptionマウス嗅覚受容体遺伝子 MOR28 をコードする遺伝子の下流に、IRES-gap-EGFP を knock-in したもので、MOR28 を発現する嗅細胞が膜結合型EGFPで可視化される。
Colony maintenance兄妹交配特記；ホモ×ホモ
ReferencesGenes Cells, 6, 71-78 (2001). 11277098
Nat. Neurosci., 3, 687-693 (2000). 10862701

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Olfr1507olfactory receptor 150714Olfr1507<tm1(EGFP)Hsak>targeted mutation 1(EGFP), Hitoshi Sakano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)14

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)14internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gap43growth associated protein 43UNGap43B-50, GAP-43, Gap43, neuromodulin

Ordering Information

供与核酸Mouse Olfr1507(MOR28), Encephalomyocarditis virus ires (internal ribosome entry site), Mouse gap43 palmitoylation signal, Jellyfish EGFP cDNA, Phage P1 loxP site
Research applicationCell Biology Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Neurosci., 3, 687-693 (2000). Genes Cells, 6, 71-78 (2001).
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor坂野　仁（国立大学法人東京大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Low VF, Mombaerts P.
Odorant receptor proteins in the mouse main olfactory epithelium and olfactory bulb.
Neuroscience 344 167-177(2017) 28057532

Zapiec B, Mombaerts P.
Multiplex assessment of the positions of odorant receptor-specific glomeruli in the mouse olfactory bulb by serial two-photon tomography.
Proc Natl Acad Sci U S A 112(43) E5873-82(2015) 26450880