Strain Information | |
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Image | |
BRC No. | RBRC02817 |
Type | Transgene Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Tg(FucciS/G2/M)#474Bsi Tg(FucciG1)#610Bsi |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N. |
Strain description | Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown). |
Colony maintenance | #474: Carrier x Noncarrier; #610: Carrier x Nocarrier [Crossing with #474 (RBRC02704) and #610 (RBRC02708)] |
References | Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. Sakaue-Sawano A, Hoshida T, Yo M, Takahashi R, Ohtawa K, Arai T, Takahashi E, Noda S, Miyoshi H, Miyawaki A Development, 140(22):4624-32 (2013). doi:10.1242/dev.099226 24154524Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system. Tomura M, Sakaue-Sawano A, Mori Y, Takase-Utsugi M, Hata A, Ohtawa K, Kanagawa O, Miyawaki A PLoS One, 8(9):e73801 (2013). 24066072 |
Health Report | |
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Examination Date / Room / Rack | 2024/10/21Room:3-CRack:F 2024/07/22Room:3-CRack:F |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
CDT1 | chromatin licensing and DNA replication factor (human) | UN | CDT1 | ||||
GMNN | geminin (human) | UN | GMNN | ||||
mAG | monomeric Azami Green (Coral) | UN | mAG | ||||
mKO2 | monomeric Kusabira Orange (Coral) | UN | mKO2 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter) rabbit beta-globin poly A, Coral mKO2 (monomeric Kusabira Orange) cDNA, human Cdt1 cDNA, Coral mAG(monomeric Azami Green), human Geminin cDNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 8(9):e73801 (2013). Development, 140(22):4624-32 (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Atsushi Miyawaki (RIKEN CBS) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- S_G2_M-Green.dna G1-Red.dna |
BRC mice in Publications |
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Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H. Fucci-guided purification of hematopoietic stem cells with high repopulating activity. Biochem Biophys Res Commun 457(1) 7-11(2015) 25534850 |