Strain Data Sheet

RBRC02759

update : 2023-05-22

Information1
Image
BRC No.	RBRC02759
Type	Targeted Mutation Congenic
Species	Mus musculus
Strain name	B6.Cg-Atg7<tm1Tchi>
Former Common name	C57BL/6-Atg7 flox/flox
H-2 Haplotype
ES Cell line	TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance	black [a/a B/B C/C]
Strain development	Developed by Masaaki Komatsu and Tomoyuki Chiba, Tokyo Metropolitan Institute of Medical Science. The targeting construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mice were backcrossed to C57BL/6 over 5 times.
Strain description	Atg7, autophagy-related 7 gene floxed mice. A targeting vector was constructed by insertion of a loxP site within introns 13 and 14. Exon 14 was fused to a cDNA fragment encoded by exons 15, 16 and 17 and polyA after the stop codon. Atg7 is essential for ATG conjugation, LC3 modification systems, and autophagosome formation. Conditional Atg7 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance	Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
References	J. Cell Biol., 169, 425-434 (2005) 15866887

Health Report
Examination Date / Room / Rack	2023/04/10Room:3-BRack:K 2023/01/10Room:3-BRack:K 2022/10/11Room:3-BRack:K 2022/07/11Room:3-BRack:K 2022/06/13Room:3-BRack:K 2022/04/11Room:3-BRack:K 2022/01/24Room:3-BRack:K 2021/12/10Room:3-BRack:K 2021/10/26Room:3-BRack:K 2021/09/03Room:3-BRack:K 2021/08/30Room:3-BRack:K 2021/07/26Room:3-BRack:K

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Atg7autophagy-related 7 (yeast)6Atg7<tm1Tchi>targeted mutation 1, Tomaki Chiba1810013K23Rik, Apg7l Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Atg7autophagy-related 7 (yeast)6Atg7<tm1Tchi>targeted mutation 1, Tomaki Chiba1810013K23Rik, Apg7l

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

neoneomycin resistance gene (E. coli)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2
Donor DNA	Polyoma enhancer/herpes simplex virus thymidine kinase gene (MC1) promoter, E. coli Neomycin resistance gene, Phage P1 loxP sites, Mouse Atg7 genomic DNA
Research application
Specific Term and Conditions	The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Blol., 169, 425-434 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE, Who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.
Depositor	Masaaki Komatsu (Tokyo Metropolitan Institute of Medical Science)
Strain Status	Live mice Frozen embryos Frozen sperm
Strain Availability	Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months)
Additional Info.	Mouse of the Month Jun 2012, Mouse of the Month Oct 2016 Genotyping protocol -PCR- Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP (Japanese)

BRC mice in Publications

Luo P, Yan H, Du J, Chen X, Shao J, Zhang Y, Xu Z, Jin Y, Lin N, Yang B, He Q. PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1). Autophagy 1-17(2020) 33315519 Muralimanoharan S, Gao X, Weintraub S, Myatt L, Maloyan A. Sexual dimorphism in activation of placental autophagy in obese women with evidence for fetal programming from a placenta-specific mouse model. Autophagy 12(5) 752-69(2016) 26986453 Wang Y, Liu X, Zhu L, Li W, Li Z, Lu X, Liu J, Hua W, Zhou Y, Gu Y, Zhu M. PG545 alleviates diabetic retinopathy by promoting retinal Müller cell autophagy to inhibit the inflammatory response. Biochem Biophys Res Commun 531(4) 452-458(2020) 32800548 Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research. Cell 140(3) 313-26(2010) 20144757 Song ZH, Yu HY, Wang P, Mao GK, Liu WX, Li MN, Wang HN, Shang YL, Liu C, Xu ZL, Sun QY, Li W. Germ cell-specific Atg7 knockout results in primary ovarian insufficiency in female mice. Cell Death Dis 6 e1589(2015) 25590799 Walter KM, Schönenberger MJ, Trötzmüller M, Horn M, Elsässer HP, Moser AB, Lucas MS, Schwarz T, Gerber PA, Faust PL, Moch H, Köfeler HC, Krek W, Kovacs WJ. Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy. Cell Metab 20(5) 882-897(2014) 25440060 Wang H, Wan H, Li X, Liu W, Chen Q, Wang Y, Yang L, Tang H, Zhang X, Duan E, Zhao X, Gao F, Li W. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res 24(7) 852-69(2014) 24853953 Bang S, Lee GK, Shin H, Suh CS, Lim HJ. Vitrification, in vitro fertilization, and development of Atg7 deficient mouse oocytes. Clin Exp Reprod Med 43(1) 9-14(2016) 27104152 Chatterji P, Williams PA, Whelan KA, Samper FC, Andres SF, Simon LA, Parham LR, Mizuno R, Lundsmith ET, Lee DS, Liang S, Wijeratne HS, Marti S, Chau L, Giroux V, Wilkins BJ, Wu GD, Shah P, Tartaglia GG, Hamilton KE. Posttranscriptional regulation of colonic epithelial repair by RNA binding protein IMP1/IGF2BP1. EMBO Rep 20(6) (2019) 31061170 Kang X, Yang W, Feng D, Jin X, Ma Z, Qian Z, Xie T, Li H, Liu J, Wang R, Li F, Li D, Sun H, Wu S. Cartilage-Specific Autophagy Deficiency Promotes ER Stress and Impairs Chondrogenesis in PERK-ATF4-CHOP-Dependent Manner. J Bone Miner Res 32(10) 2128-2141(2017) 28304100 Li KC, Wang CH, Zou JJ, Qu C, Wang XL, Tian XS, Liu HW, Cui T. Loss of Atg7 in Endothelial Cells Enhanced Cutaneous Wound Healing in a Mouse Model. J Surg Res 249 145-155(2020) 31958599 Zhang Y, Cross SD, Stanton JB, Marmorstein AD, Le YZ, Marmorstein LY. Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of Atg5 or Atg7. Mol Vis 23 228-241(2017) 28465655 Luciani A, Schumann A, Berquez M, Chen Z, Nieri D, Failli M, Debaix H, Festa BP, Tokonami N, Raimondi A, Cremonesi A, Carrella D, Forny P, Kölker S, Diomedi Camassei F, Diaz F, Moraes CT, Di Bernardo D, Baumgartner MR, Devuyst O. Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency. Nat Commun 11(1) 970(2020) 32080200

BRC mice in Publications

Luo P, Yan H, Du J, Chen X, Shao J, Zhang Y, Xu Z, Jin Y, Lin N, Yang B, He Q.
PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1).
Autophagy 1-17(2020) 33315519

Muralimanoharan S, Gao X, Weintraub S, Myatt L, Maloyan A.
Sexual dimorphism in activation of placental autophagy in obese women with evidence for fetal programming from a placenta-specific mouse model.
Autophagy 12(5) 752-69(2016) 26986453

Wang Y, Liu X, Zhu L, Li W, Li Z, Lu X, Liu J, Hua W, Zhou Y, Gu Y, Zhu M.
PG545 alleviates diabetic retinopathy by promoting retinal Müller cell autophagy to inhibit the inflammatory response.
Biochem Biophys Res Commun 531(4) 452-458(2020) 32800548

Mizushima N, Yoshimori T, Levine B.
Methods in mammalian autophagy research.
Cell 140(3) 313-26(2010) 20144757

Song ZH, Yu HY, Wang P, Mao GK, Liu WX, Li MN, Wang HN, Shang YL, Liu C, Xu ZL, Sun QY, Li W.
Germ cell-specific Atg7 knockout results in primary ovarian insufficiency in female mice.
Cell Death Dis 6 e1589(2015) 25590799

Walter KM, Schönenberger MJ, Trötzmüller M, Horn M, Elsässer HP, Moser AB, Lucas MS, Schwarz T, Gerber PA, Faust PL, Moch H, Köfeler HC, Krek W, Kovacs WJ.
Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.
Cell Metab 20(5) 882-897(2014) 25440060

Wang H, Wan H, Li X, Liu W, Chen Q, Wang Y, Yang L, Tang H, Zhang X, Duan E, Zhao X, Gao F, Li W.
Atg7 is required for acrosome biogenesis during spermatogenesis in mice.
Cell Res 24(7) 852-69(2014) 24853953

Bang S, Lee GK, Shin H, Suh CS, Lim HJ.
Vitrification, in vitro fertilization, and development of Atg7 deficient mouse oocytes.
Clin Exp Reprod Med 43(1) 9-14(2016) 27104152

Chatterji P, Williams PA, Whelan KA, Samper FC, Andres SF, Simon LA, Parham LR, Mizuno R, Lundsmith ET, Lee DS, Liang S, Wijeratne HS, Marti S, Chau L, Giroux V, Wilkins BJ, Wu GD, Shah P, Tartaglia GG, Hamilton KE.
Posttranscriptional regulation of colonic epithelial repair by RNA binding protein IMP1/IGF2BP1.
EMBO Rep 20(6) (2019) 31061170

Kang X, Yang W, Feng D, Jin X, Ma Z, Qian Z, Xie T, Li H, Liu J, Wang R, Li F, Li D, Sun H, Wu S.
Cartilage-Specific Autophagy Deficiency Promotes ER Stress and Impairs Chondrogenesis in PERK-ATF4-CHOP-Dependent Manner.
J Bone Miner Res 32(10) 2128-2141(2017) 28304100

Li KC, Wang CH, Zou JJ, Qu C, Wang XL, Tian XS, Liu HW, Cui T.
Loss of Atg7 in Endothelial Cells Enhanced Cutaneous Wound Healing in a Mouse Model.
J Surg Res 249 145-155(2020) 31958599

Zhang Y, Cross SD, Stanton JB, Marmorstein AD, Le YZ, Marmorstein LY.
Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of Atg5 or Atg7.
Mol Vis 23 228-241(2017) 28465655

Luciani A, Schumann A, Berquez M, Chen Z, Nieri D, Failli M, Debaix H, Festa BP, Tokonami N, Raimondi A, Cremonesi A, Carrella D, Forny P, Kölker S, Diomedi Camassei F, Diaz F, Moraes CT, Di Bernardo D, Baumgartner MR, Devuyst O.
Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency.
Nat Commun 11(1) 970(2020) 32080200

Information1

Health Report

Gene

Information2

BRC mice in Publications