Strain Information | |
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Image | |
BRC No. | RBRC02759 |
Type | Targeted Mutation Congenic![]() |
Species | Mus musculus |
Strain name | B6.Cg-Atg7<tm1Tchi> |
Former Common name | C57BL/6-Atg7 flox/flox |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Masaaki Komatsu and Tomoyuki Chiba, Tokyo Metropolitan Institute of Medical Science. The targeting construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mice were backcrossed to C57BL/6 over 5 times. |
Strain description | Atg7, autophagy-related 7 gene floxed mice. A targeting vector was constructed by insertion of a loxP site within introns 13 and 14. Exon 14 was fused to a cDNA fragment encoded by exons 15, 16 and 17 and polyA after the stop codon. Atg7 is essential for ATG conjugation, LC3 modification systems, and autophagosome formation. Conditional Atg7 deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile. |
References | Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. Komatsu M, Waguri S, Ueno T, Iwata J, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Uchiyama Y, Kominami E, Tanaka K, Chiba T J. Cell Biol., 169, 425-434 (2005) 15866887 |
Health Report | |
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Examination Date / Room / Rack | 2024/04/08Room:3-BRack:K 2024/01/09Room:3-BRack:K 2023/10/10Room:3-BRack:K 2023/07/10Room:3-BRack:K 2023/06/26Room:3-BRack:K 2023/06/26Room:3-BRack:K 2023/04/10Room:3-BRack:K 2023/01/10Room:3-BRack:K 2022/10/11Room:3-BRack:K |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Atg7 | autophagy related 7 | 6 | Atg7 | targeted mutation 1, Tomaki Chiba | |||
loxP | phage P1 loxP | 6 | loxP | ||||
loxP | phage P1 loxP | 6 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 6 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 29 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Polyoma enhancer/herpes simplex virus thymidine kinase gene (MC1) promoter, E. coli Neomycin resistance gene, Phage P1 loxP sites, Mouse Atg7 genomic DNA |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Blol., 169, 425-434 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE, Who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Masaaki Komatsu (Tokyo Metropolitan Institute of Medical Science) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2012, Mouse of the Month Oct 2016 |
BRC mice in Publications |
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Williams PA, Naughton KE, Simon LA, Soto GE, Parham LR, Ma X, Danan CH, Hu W, Friedman ES, McMillan EA, Mehta H, Stoltz MA, Soto Ocaña J, Zackular J, Bittinger K, Whelan KA, Karakasheva TA, Hamilton KE. Intestinal epithelial autophagy is required for the regenerative benefit of calorie restriction. Am J Physiol Gastrointest Liver Physiol (2023) 36852920 |
Luo P, Yan H, Du J, Chen X, Shao J, Zhang Y, Xu Z, Jin Y, Lin N, Yang B, He Q. PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1). Autophagy 17(10) 3221-3237(2021) 33315519 |
Muralimanoharan S, Gao X, Weintraub S, Myatt L, Maloyan A. Sexual dimorphism in activation of placental autophagy in obese women with evidence for fetal programming from a placenta-specific mouse model. Autophagy 12(5) 752-69(2016) 26986453 |
Wang Y, Liu X, Zhu L, Li W, Li Z, Lu X, Liu J, Hua W, Zhou Y, Gu Y, Zhu M. PG545 alleviates diabetic retinopathy by promoting retinal Müller cell autophagy to inhibit the inflammatory response. Biochem Biophys Res Commun 531(4) 452-458(2020) 32800548 |
Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research. Cell 140(3) 313-26(2010) 20144757 |
Song ZH, Yu HY, Wang P, Mao GK, Liu WX, Li MN, Wang HN, Shang YL, Liu C, Xu ZL, Sun QY, Li W. Germ cell-specific Atg7 knockout results in primary ovarian insufficiency in female mice. Cell Death Dis 6(1) e1589(2015) 25590799 |
Yan B, Belke D, Gui Y, Chen YX, Jiang ZS, Zheng XL. Pharmacological inhibition of MALT1 (mucosa-associated lymphoid tissue lymphoma translocation protein 1) induces ferroptosis in vascular smooth muscle cells. Cell Death Discov 9(1) 456(2023) 38097554 |
Walter KM, Schönenberger MJ, Trötzmüller M, Horn M, Elsässer HP, Moser AB, Lucas MS, Schwarz T, Gerber PA, Faust PL, Moch H, Köfeler HC, Krek W, Kovacs WJ. Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy. Cell Metab 20(5) 882-897(2014) 25440060 |
Klochkova A, Karami AL, Fuller AD, Parham LR, Panchani SR, Natarajan S, Jackson JL, Mu A, Tan Y, Cai KQ, Klein-Szanto AJ, Muir AB, Tétreault MP, Graña X, Hamilton KE, Whelan KA. Autophagy Contributes to Homeostasis in Esophageal Epithelium Where High Autophagic Vesicle Level Marks Basal Cells With Limited Proliferation and Enhanced Self-Renewal Potential. Cell Mol Gastroenterol Hepatol 18(1) 15-40(2024) 38452871 |
Parham LR, Williams PA, Katada K, Nettleford SK, Chatterji P, Acheampong KK, Danan CH, Ma X, Simon LA, Naughton KE, Karakasheva T, McMillan EA, Whelan KA, Brady DC, Shaffer SM, Hamilton KE. IGF2BP1/IMP1 deletion enhances a facultative stem cell state via regulation of MAP1LC3B. Cell Mol Gastroenterol Hepatol (2023) 38081361 |
Wang H, Wan H, Li X, Liu W, Chen Q, Wang Y, Yang L, Tang H, Zhang X, Duan E, Zhao X, Gao F, Li W. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res 24(7) 852-69(2014) 24853953 |
Bang S, Lee GK, Shin H, Suh CS, Lim HJ. Vitrification, in vitro fertilization, and development of Atg7 deficient mouse oocytes. Clin Exp Reprod Med 43(1) 9-14(2016) 27104152 |
Chatterji P, Williams PA, Whelan KA, Samper FC, Andres SF, Simon LA, Parham LR, Mizuno R, Lundsmith ET, Lee DS, Liang S, Wijeratne HS, Marti S, Chau L, Giroux V, Wilkins BJ, Wu GD, Shah P, Tartaglia GG, Hamilton KE. Posttranscriptional regulation of colonic epithelial repair by RNA binding protein IMP1/IGF2BP1. EMBO Rep 20(6) (2019) 31061170 |
Kang X, Yang W, Feng D, Jin X, Ma Z, Qian Z, Xie T, Li H, Liu J, Wang R, Li F, Li D, Sun H, Wu S. Cartilage-Specific Autophagy Deficiency Promotes ER Stress and Impairs Chondrogenesis in PERK-ATF4-CHOP-Dependent Manner. J Bone Miner Res 32(10) 2128-2141(2017) 28304100 |
Li KC, Wang CH, Zou JJ, Qu C, Wang XL, Tian XS, Liu HW, Cui T. Loss of Atg7 in Endothelial Cells Enhanced Cutaneous Wound Healing in a Mouse Model. J Surg Res 249 145-155(2020) 31958599 |
Zhang Y, Cross SD, Stanton JB, Marmorstein AD, Le YZ, Marmorstein LY. Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of Atg5 or Atg7. Mol Vis 23 228-241(2017) 28465655 |
Song Y, Na H, Lee SE, Kim YM, Moon J, Nam TW, Ji Y, Jin Y, Park JH, Cho SC, Lee J, Hwang D, Ha SJ, Park HW, Kim JB, Lee HW. Dysfunctional adipocytes promote tumor progression through YAP/TAZ-dependent cancer-associated adipocyte transformation. Nat Commun 15(1) 4052(2024) 38744820 |
Tan HWS, Lu G, Dong H, Cho YL, Natalia A, Wang L, Chan C, Kappei D, Taneja R, Ling SC, Shao H, Tsai SY, Ding WX, Shen HM. A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery. Nat Commun 13(1) 3720(2022) 35764633 |
Luciani A, Schumann A, Berquez M, Chen Z, Nieri D, Failli M, Debaix H, Festa BP, Tokonami N, Raimondi A, Cremonesi A, Carrella D, Forny P, Kölker S, Diomedi Camassei F, Diaz F, Moraes CT, Di Bernardo D, Baumgartner MR, Devuyst O. Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency. Nat Commun 11(1) 970(2020) 32080200 |