Strain Information | |
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Image | |
BRC No. | RBRC02704 |
Type | Transgene Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Tg(FucciS/G2/M)#474Bsi |
Former Common name | B6.Cg-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N. |
Strain description | Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown). |
Colony maintenance | Carrier x Carrier [or Crossing to C57BL/6NJcl] |
References | Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. Sakaue-Sawano A, Hoshida T, Yo M, Takahashi R, Ohtawa K, Arai T, Takahashi E, Noda S, Miyoshi H, Miyawaki A Development 140 (22): 4624-4632 (2013). 24154524Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system. Tomura M, Sakaue-Sawano A, Mori Y, Takase-Utsugi M, Hata A, Ohtawa K, Kanagawa O, Miyawaki A PLoS One, 8(9):e73801 (2013). 24066072Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A Cell, 132, 487-498 (2008). 18267078 |
Health Report | |
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Examination Date / Room / Rack | 2024/10/21Room:3-CRack:A 2024/07/22Room:3-CRack:A 2024/04/22Room:3-CRack:A 2024/01/22Room:3-CRack:A 2023/10/23Room:3-CRack:A 2023/07/24Room:3-CRack:A 2023/04/24Room:3-CRack:A 2023/01/23Room:3-CRack:A |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
GMNN | geminin (human) | UN | GMNN | ||||
mAG | monomeric Azami Green (Coral) | UN | mAG | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Coral mAG(monomeric Azami Green) cDNA, human Geminin cDNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 8(9):e73801 (2013). Development 140 (22): 4624-4632 (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Atsushi Miyawaki (RIKEN CBS) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- S_G2_M-Green.dna |
BRC mice in Publications |
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Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H. Fucci-guided purification of hematopoietic stem cells with high repopulating activity. Biochem Biophys Res Commun 457(1) 7-11(2015) 25534850 |
Thion MS, Low D, Silvin A, Chen J, Grisel P, Schulte-Schrepping J, Blecher R, Ulas T, Squarzoni P, Hoeffel G, Coulpier F, Siopi E, David FS, Scholz C, Shihui F, Lum J, Amoyo AA, Larbi A, Poidinger M, Buttgereit A, Lledo PM, Greter M, Chan JKY, Amit I, Beyer M, Schultze JL, Schlitzer A, Pettersson S, Ginhoux F, Garel S. Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner. Cell 172(3) 500-516.e16(2018) 29275859 |
Evrard M, Kwok IWH, Chong SZ, Teng KWW, Becht E, Chen J, Sieow JL, Penny HL, Ching GC, Devi S, Adrover JM, Li JLY, Liong KH, Tan L, Poon Z, Foo S, Chua JW, Su IH, Balabanian K, Bachelerie F, Biswas SK, Larbi A, Hwang WYK, Madan V, Koeffler HP, Wong SC, Newell EW, Hidalgo A, Ginhoux F, Ng LG. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity 48(2) 364-379.e8(2018) 29466759 |
Takegahara N, Kim H, Mizuno H, Sakaue-Sawano A, Miyawaki A, Tomura M, Kanagawa O, Ishii M, Choi Y. Involvement of Receptor Activator of Nuclear Factor-κB Ligand (RANKL)-induced Incomplete Cytokinesis in the Polyploidization of Osteoclasts. J Biol Chem 291(7) 3439-54(2016) 26670608 |
Shand FH, Ueha S, Otsuji M, Koid SS, Shichino S, Tsukui T, Kosugi-Kanaya M, Abe J, Tomura M, Ziogas J, Matsushima K. Tracking of intertissue migration reveals the origins of tumor-infiltrating monocytes. Proc Natl Acad Sci U S A 111(21) 7771-6(2014) 24825888 |
Teh YC, Chooi MY, Liu D, Kwok I, Lai GC, Ayub Ow Yong L, Ng M, Li JLY, Tan Y, Evrard M, Tan L, Liong KH, Leong K, Goh CC, Chan AYJ, Shadan NB, Mantri CK, Hwang YY, Cheng H, Cheng T, Yu W, Tey HL, Larbi A, St John A, Angeli V, Ruedl C, Lee B, Ginhoux F, Chen SL, Ng LG, Ding JL, Chong SZ. Transitional premonocytes emerge in the periphery for host defense against bacterial infections. Sci Adv 8(9) eabj4641(2022) 35245124 |