Strain Data Sheet
RBRC02657
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC02657 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito> |
| Former Common name | ROSA nls LacZ |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | 理化学研究所脳科学総合研究センター・糸原重美先生。E14 ES細胞由来。C57BL/6Jコンジェニック。 |
| Strain description | Gt(RSA)26Sor遺伝子座にNLS-lacZをノックインしたレポーターマウス。組織特異的Cre発現マウスとの交配によりlacZを発現する。核がlacZにより可視化され、細胞体の局在が観察可能。ホモマウスは外見上特に異常は認められない。 |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | Front. Behav. Neurosci., 7:17 (2013). 23487260 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/01/09Room:3-ARack:K 2023/10/10Room:3-ARack:K 2023/07/10Room:3-ARack:K 2023/04/10Room:3-ARack:K 2023/01/10Room:3-ARack:K 2022/10/11Room:3-ARack:K 2022/07/11Room:3-ARack:K |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito>targeted mutation 1, Shigeyoshi Itohara Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 nuclear localaization signal (chemically-synthesized nucleotide)6nls |
Ordering Information | |
|---|---|
| 供与核酸 | synthetic splice acceptor sequence, P1 phage loxP sites, STOP (mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neo, polyA), Simian virus 40 Large T antigen nuclear localization signal (NLS), E.coli lacZ, SV40 polyA signal, mouse ROSA26 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| 提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。Front. Behav. Neurosci., 7:17, (2013). |
| Depositor | 糸原 重美（独立行政法人理化学研究所） |
| Strain Status |  生体 凍結胚 凍結精子 |
| Strain Availability | 凍結精子を1ヶ月以内に提供可能 生体マウスを1~3ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Mar 2013 |
BRC mice in Publications |
|---|
Namiki T, Kamoshita M, Kageyama A, Terakawa J, Ito J, Kashiwazaki N. Utility of progesterone receptor-ires-Cre to generate conditional knockout mice for uterine study. Anim Sci J 92(1) e13615(2021) 34402137 Yoshimura Y, Ida-Tanaka M, Hiramaki T, Goto M, Kamisako T, Eto T, Yagoto M, Kawai K, Takahashi T, Nakayama M, Ito M. Novel reporter and deleter mouse strains generated using VCre/VloxP and SCre/SloxP systems, and their system specificity in mice. Transgenic Res 27(2) 193-201(2018) 29546522 |