Strain Data Sheet

RBRC02655

Strain Information

Image
BRC No.RBRC02655
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;129-Xist<tm5Sado>
Former Common nameXist<delta A>; B6.Cg-Xist<tm5Sado>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2007. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6).
Strain descriptionXist<delta> mice. The portion of exon 1 encoding the A repeat was deleted. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
References
A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse.
Yuko Hoki, Naomi Kimura, Minako Kanbayashi, Yuko Amakawa, Tatsuya Ohhata, Hiroyuki Sasaki, Takashi Sado
Development, 136 (1) 139-146 (2009). 19036803

Health Report

Examination Date / Room / Rack2025/12/22Room:4-ARack:DSentinel mouse program
2025/10/14Room:4-ARack:DSentinel mouse program
2025/07/28Room:4-ARack:DSentinel mouse program
2025/05/19Room:4-ARack:DSentinel mouse program
2025/02/17Room:4-ARack:DSentinel mouse program
2024/11/18Room:4-ARack:DSentinel mouse program
2024/08/19Room:4-ARack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Xist
MGI:98974		inactive X specific transcriptsXXist<tm5Sado>
MGI:3829654		targeted mutation 5, Takashi Sado
loxP phage P1 loxPXloxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal embryo development (MP:0001672)

  • abnormal extraembryonic ectoderm morphology (MP:0010736)

  • abnormal imprinting (MP:0003787)

  • decreased embryo size (MP:0001698)

  • prenatal lethality, incomplete penetrance (MP:0011101)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Xist genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
    DepositorTakashi Sado (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2009

    BRC mice in Publications

    Hoelzl S, Hasenbein TP, Engelhardt S, Andergassen D.
    Aging promotes reactivation of the Barr body at distal chromosome regions.
    Nat Aging (2025) 40312568
    Lemoine L, Hoelzl S, Hasenbein TP, Graf E, Andergassen D.
    Long-read sequencing disentangles isoform complexity at allele-specific loci.
    Sci Rep 15(1) 39389(2025) 41219345
    Fukuda A, Mitani A, Miyashita T, Sado T, Umezawa A, Akutsu H.
    Maintenance of Xist Imprinting Depends on Chromatin Condensation State and Rnf12 Dosage in Mice.
    PLoS Genet 12(10) e1006375(2016) 27788132