Strain Data Sheet

RBRC02599

Strain Information

Image
BRC No.RBRC02599
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameIVCS.129P2(B6)-Akr1c18<tm1Mni>
Former Common name20alpha-HSD knock-out mouse
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainIVCS
Appearance
Strain development開発者／機関：東京大学大学院農学生命科学研究科獣医生理学教室および東京大学医科学研究所ヒト疾患モデル研究センターとの共同研究により作出開発年：2001年
Strain descriptionIVCSを背景に20alpha-HSD遺伝子を欠損したマウス
Colony maintenance戻し交配（C57BL/6jに7回戻し交配後、IVCSに12回戻し交配）
ReferencesJ. Reprod. Dev., 53, 499-508 (2007) 17272929

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Akr1c18aldo-keto reductase family 1, member C1813Akr1c18<tm1Mni>targeted mutation 1, Masugi Nishihara20alpha-HSD, 20alpha-hydroxysteroid dehydrogenase

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)13

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)13internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish EGFP cDNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine Growth Hormone poly-A signal,mouse 20 alpha-hydroxysteroid dehydrogenase genomic DNA
Research applicationFluorescent Proteins/lacZ System
Reproductive Biology Research
提供条件条件を付加しない。
Depositor西原　眞杉（国立大学法人東京大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data