Strain Data Sheet

RBRC02594

Strain Information

Image
BRC No.RBRC02594
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Sept3<tm1Keng>/SiaRbrc
Former Common nameSept3(-) (Acc. No. CDB0422K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Mineko Kengaku, RIKEN Brain Science Institute. A neomycin resistance cassette flanked by loxP sites was transferred into TT2 ES cells to replace a partial region of exon 2 and exons 3-5 of Sept3 gene.
Strain descriptionSept3 knock-out mice. Septins are a family of GTPase proteins that are required for cytokinesis in most eukaryotes. Sept3 is a brain-specific protein that is abundant in synaptic terminals. Sept3 knockout mutant mice show normal histogenesis and neuronal differentiation.
Colony maintenance
ReferencesJ. Neurochem., 102, 77-92 (2007) 17564677

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sept3septin 315Sept3<tm1Keng>targeted mutation 1, Mineko Kengaku3110018K01Rik, B530002E20Rik, Sep3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sept3 genomic DNA
Research applicationCre/loxP system
Neurobiology Research
提供条件条件を付加する。
(1) 分与希望者は事前に見学美根子（kengaku@brain.riken.jp) に連絡をとり、分与に関して許可を得ること。 (2) 当該マウスを用いる論文発表に際しては、Acc. No. CDB0422K とともに当該マウスを記載すること。(3) 当該マウス用いる論文発表に際してはその由来として第一報論文 Journal of Neurochemistry, 102, 77-92 (2007). を引用すること。(4) 発表論文を寄託者 (mutant.bdr@riken.jp) に連絡すること。
DepositorRIKEN CDB（独立行政法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

No Data