Strain Data Sheet

RBRC02584

update : 2025-06-20

Strain Information
Image
BRC No.	RBRC02584
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6;129-Drd1a<tm1Mok> Drd2<tm1Mok> Drd3<tm1Mok> Drd4<tm1Mok> Drd5<tm1Mok>
Former Common name	D1RD2RD3RD4RD5R, D1R/D2R/D3R/D4R/D5R KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development	Developed by Motoya Katsuki, National Institutes of Natural Sciences.
Strain description	D1R (Drd1a), D2R (Drd2), D3R (Drd3) and D4R (Drd4) multiple knockout mice.
Colony maintenance
References	Dopamine D2 receptor plays a critical role in cell proliferation and proopiomelanocortin expression in the pituitary. Yamaguchi H, Aiba A, Nakamura K, Nakao K, Sakagami H, Goto K, Kondo H, Katsuki M Genes Cells. 1(2):253-68 (1996). 9140068 Dopamine D1 receptor modulates hippocampal representation plasticity to spatial novelty. Tran A H, Uwano T, Kimura T, Hori E, Katsuki M, Nishijo H, Ono T J. Neurosci., 28(50):13390-13400 (2008). 19074012 Methamphetamine increases locomotion and dopamine transporter activity in dopamine d5 receptor-deficient mice. Hayashizaki S, Hirai S, Ito Y, Honda Y, Arime Y, Sora I, Okado H, Kodama T, Takada M PLoS One, 8(10):e75975 (2013). 24155877

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Drd1 MGI:99578	dopamine receptor D1	13	Drd1<tm1Mok> MGI:3829942	targeted mutation 1, Motoya Katsuki
Ecogpt	E.coli xanthine phosphoribosyltransferase	13	Ecogpt			mouse phosphoglycerate kinase promoter (PGK promoter)
neo	neomycin resistance gene (E. coli)	13				herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Drd3 MGI:94925	dopamine receptor D3	16	Drd3<tm1Mok> MGI:6376625	targeted mutation 1, Motoya Katsuki			schizophrenia(MedGEN) tremor, hereditary essential, 1(MedGEN)
Ecogpt	E.coli xanthine phosphoribosyltransferase	16	Ecogpt			mouse phosphoglycerate kinase promoter (PGK promoter)
neo	neomycin resistance gene (E. coli)	16				herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Drd5 MGI:94927	dopamine receptor D5	5	Drd5<tm1Mok> MGI:5569103	targeted mutation 1, Motoya Katsuki			attention deficit-hyperactivity disorder(MedGEN) benign essential blepharospasm(MedGEN)
Ecogpt	E.coli xanthine phosphoribosyltransferase	5	Ecogpt			mouse phosphoglycerate kinase promoter (PGK promoter)
neo	neomycin resistance gene (E. coli)	5				herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Drd4 MGI:94926	dopamine receptor D4	7	Drd4<tm1Mok> MGI:6376626	targeted mutation 1, Motoya Katsuki			attention deficit-hyperactivity disorder(MedGEN)
Ecogpt	E.coli xanthine phosphoribosyltransferase	7	Ecogpt			mouse phosphoglycerate kinase promoter (PGK promoter)
neo	neomycin resistance gene (E. coli)	7				mouse RNA polymerase II large subunit promoter
Drd2 MGI:94924	dopamine receptor D2	9	Drd2<tm1Mok> MGI:3576789	targeted mutation 1, Motoya Katsuki
neo	neomycin resistance gene (E. coli)	9				herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype
Annotation by Mammalian phenotyhpe ontology	abnormal circulating hormone level(MP:0005418) abnormal single cell response(MP:0003463) abnormal synaptic plasticity(MP:0004859) darkened coat color(MP:0005409) enlarged pituitary intermediate lobe(MP:0008370) more 1 phenotypes increased pituitary hormone level(MP:0003973)
Detailed phenotype data

Ordering Information
Donor DNA	Dopamine receptor1,2,3,4,5,E. coli neo, xanthine phosphoribosyltransferase (Ecogpt)
Research application	Neurobiology Research
Specific Term and Conditions	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 28, 13390-13400 (2008). Genes Cells, 1, 253-268 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Depositor	Toshikuni Sasaoka (Niigata University)
Strain Status	Frozen embryos
Strain Availability	Recovery and QC required prior to distribution
Additional Info.	Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data