Strain Data Sheet

RBRC02523

update : 2025-03-19

Strain Information
Image
BRC No.	RBRC02523
Type	Targeted Mutation Congenic
Species	Mus musculus
Strain name	B6.Cg-Drd1a<tm1Mok> Drd2<tm1Mok> Drd1a<tm1(tTA)Mok> Tg(TRE-Drd1a/lacZ)1Mok
Former Common name	D1R, TET, D1R, D2R KO, B6-D1R/D2R KO D1R/tTA TRE-D1R/lacZ
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development	Developed by Motoya Katsuki, National Institutes of Natural Sciences.
Strain description	D1R (Drd1a) knockout, D2R (Drd2) knockout, D1R-tTA knock-in and TRE-D1R/lacZ transgene multiple mutant mice.
Colony maintenance
References	Dopamine D2 receptor plays a critical role in cell proliferation and proopiomelanocortin expression in the pituitary. Yamaguchi H, Aiba A, Nakamura K, Nakao K, Sakagami H, Goto K, Kondo H, Katsuki M Genes Cells. 1(2):253-68 (1996). 9140068 Dopamine D1 receptor modulates hippocampal representation plasticity to spatial novelty. Tran A H, Uwano T, Kimura T, Hori E, Katsuki M, Nishijo H, Ono T J. Neurosci., 28(50):13390-13400 (2008). 19074012 Distinct motor impairments of dopamine D1 and D2 receptor knockout mice revealed by three types of motor behavior. Nakamura T, Sato A, Kitsukawa T, Momiyama T, Yamamori T, Sasaoka T Front Integr Neurosci., 8:56 (2014). 25076876 Expression pattern of immediate early genes in the cerebellum of D1R KO, D2R KO, and wild type mice under vestibular-controlled activity. Nakamura T, Sato A, Kitsukawa T, Sasaoka T, Yamamori T Front Cell Dev Biol., 3:38 (2015). 26137459 Dopamine D1 Receptor-Mediated Transmission Maintains Information Flow Through the Cortico-Striato-Entopeduncular Direct Pathway to Release Movements. Chiken S, Sato A, Ohta C, Kurokawa M, Arai S, Maeshima J, Sunayama-Morita T, Sasaoka T, Nambu A Cerebral Cortex., 25(12):4885-97 (2015). 26443442

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Drd1 MGI:99578	dopamine receptor D1	13	Drd1<tm1Mok> MGI:3829942	targeted mutation 1, Motoya Katsuki
tTA	tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)	13	tTA
Drd2 MGI:94924	dopamine receptor D2	9	Drd2<tm1Mok> MGI:3576789	targeted mutation 1, Motoya Katsuki
Drd1 MGI:99578	dopamine receptor D1A	UN	Drd1			E. coli TRE promoter (tetracycline responsive promoter)
lacZ	beta-galactosidase (E. coli)	UN
neo	neomycin resistance gene (E. coli)	UN

Phenotype
Annotation by Mammalian phenotyhpe ontology	abnormal circulating hormone level(MP:0005418) abnormal single cell response(MP:0003463) abnormal synaptic plasticity(MP:0004859) darkened coat color(MP:0005409) enlarged pituitary intermediate lobe(MP:0008370) more 1 phenotypes increased pituitary hormone level(MP:0003973)
Detailed phenotype data

Ordering Information
Donor DNA	Dopamine receptor1, E. coli neo, xanthine phosphoribosyltransferase (Ecogpt)tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), E. coli tetracycline operator, E. coli lacZ
Research application	Fluorescent Proteins/lacZ System Neurobiology Research Tet system
Specific Term and Conditions	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 28, 13390-13400 (2008). Genes Cells, 1, 253-268 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Depositor	Toshikuni Sasaoka (Niigata University)
Strain Status	Frozen embryos
Strain Availability	Recovery and QC required prior to distribution
Additional Info.	Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data