Strain Data Sheet

RBRC02508

Information1

Image
BRC No.RBRC02508
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameΔneo-mNos2-L(N2enh-nos2(dZ)EGFP)#2
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga, National Institute of Genetics in 2007.
Strain descriptionThis mutant mouse expreses a Nanos2-EGFP fusion protein lacking a C terminal region with a zinc finger motif in a Nanos2-expressing cell on Nanos2 knockout background.
Colony maintenance
References

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nanos2nanos homolog 2 (Drosophila)UNNanos2nos2Nanos2 enhancer promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNNanos2 enhancer promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNANanos2 enhancer promoter, Nanos2 (ΔZ)EGFP LacZ 遺伝子, lox 配列
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data