Strain Data Sheet

RBRC02498

Strain Information

Image
BRC No.RBRC02498
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(Stra8-EGFP)1Ysa/YsaRbrc
Former Common nameBAC-stra8EGFP-bghpA#1
H-2 Haplotype
ES Cell line
Background strain
AppearanceUnfixed
Strain development2007年、相賀裕美子先生、国立遺伝研
Strain descriptionBAC transgenic, Stra8 遺伝子にEGFP遺伝子を融合させ、ベータグロビンのポリAシグナルを持つBAC constructを導入したマウス。
Colony maintenanceヘテロで維持
ReferencesBiol Reprod., 89(1):3 (2013). 23677978

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Stra8stimulated by retinoic acid gene 8UNStra8

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2beta-globin polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)UN

Ordering Information

供与核酸Jellyfish EGFP cDNA, beta-globin-polyA signal, mouse Stra8 genomic DNA, yeast FRT (flipase recombination target) sites, E. coli neo, DNA from BAC clone RP23-367A8
Research applicationDevelopmental Biology Research
FLP/frt system
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
寄託後、2年間については、利用者は利用内容を寄託者に連絡し、共同研究の要否に関する確認をとり、共同研究が必要な場合には共著とする。
Depositor相賀　裕美子（大学共同利用機関法人情報・システム研究機構国立遺伝学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y, Kon Y.
Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes.
Biol Reprod 89(1) 3(2013) 23677978