Strain Data Sheet

RBRC02492

Strain Information

Image
BRC No.RBRC02492
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp1/Mesp2<tm1Ysa>Tg(deltaP2deltaEME)2Ysa/YsaRbrc
Former Common nameBAC-ΔP2ΔEME#2/Mesp1 Mesp2-dko
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development2007年、相賀裕美子先生、国立遺伝研
Strain descriptionBAC transgenic, Mesp2 遺伝子座を欠損し、Mesp1の体節での発現を失ったBAC constructをMesp1, Mesp2のダブルノックアウトマウス (ヘテロ) に導入したマウス。BAC由来のMesp1は初期の中胚葉でのみ発現する.
Colony maintenanceヘテロで維持
References
Identification of presomitic mesoderm (PSM)-specific Mesp1 enhancer and generation of a PSM-specific Mesp1/Mesp2-null mouse using BAC-based rescue technology.
Masayuki Oginuma, Tatsumi Hirata, Yumiko Saga
Mech. Dev., 125, 432-440 (2008) 18328678

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mesp1/Mesp2 mesoderm posterior 17Mesp1/Mesp2<tm1Ysa>
MGI:2386200		targeted mutation 1, Yumiko Saga
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)
ALP alkaline phosphatase (human)UNALP
Frt yeast FRT (flippase recombination target) siteUNFrt

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal somite development (MP:0001688)

  • abnormal vertebral arch morphology (MP:0004599)

  • abnormal vertebral lamina morphology (MP:0004605)

  • abnormal vertebral pedicle morphology (MP:0004604)

  • abnormal vertebral transverse process morphology (MP:0004600)
    • Detailed phenotype data

    Ordering Information

    供与核酸human alkaline phosphatase-pA cDNA, yeast Frt, BAC(Bacterial artificial chromosome)clone containing mouse Mesp1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo
    Research applicationDevelopmental Biology Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Mech Dev., 125, 432-440 (2008).
    Depositor相賀　裕美子（大学共同利用機関法人情報・システム研究機構国立遺伝学研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data