Strain Data Sheet

RBRC02485

Strain Information

Image
BRC No.RBRC02485
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hras1<tm1Mok> Nras<tm1Mok> Kras<tm1Mok> Tg(HRAS/floxed)1Mok
Former Common nameH-ras, N-ras, K-ras triple KO, L-Tg, B6-Hras KO/Nras KO/Kras KO/HRASfloxed Tg
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences.
Strain descriptionHras knockout, Nras knockout, Kras knockout and Lras transgenic multiple mutant mice.
Colony maintenance
References
K-ras is essential for the development of the mouse embryo.
Koera K, Nakamura K, Nakao K, Miyoshi J, Toyoshima K, Hatta T, Otani H, Aiba A, Katsuki M
Oncogene, 15, 1151-1159 (1997). 9294608

Targeted deletion of the H-ras gene decreases tumor formation in mouse skin carcinogenesis.
Ise K, Nakamura K, Nakao K, Shimizu S, Harada H, Ichise T, Miyoshi J, Gondo Y, Ishikawa T, Aiba A, Katsuki M
Oncogene, 19, 2951-2956 (2000). 10871846

K-Ras mediates cytokine-induced formation of E-cadherin-based adherens junctions during liver development.
Matsui T, Kinoshita T, Morikawa Y, Tohya K, Katsuki M, Ito Y, Kamiya A, Miyajima A
EMBO J. 21(5):1021-30 (2002). 11867530

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Csde1
MGI:92356		cold shock domain containing E1, RNA binding3Csde1
Nras
MGI:97376		neuroblastoma ras oncogene3Nras<tm1Mok>
MGI:3794957		targeted mutation 1, Motoya Katsuki
  • autoimmune lymphoproliferative syndrome type 4(MedGEN)

  • colorectal cancer(MedGEN)

  • colorectal carcinoma(DisGeNET)
    • more 7 Diseases
  • juvenile myelomonocytic leukemia(DisGeNET)

  • large congenital melanocytic nevus(MedGEN)

  • linear nevus sebaceous syndrome(MedGEN)

  • neurocutaneous melanocytosis(MedGEN)

  • nevus, epidermal(MedGEN)

  • Noonan syndrome 6(MedGEN)

  • thyroid cancer, nonmedullary, 2(MedGEN)
    • neo neomycin resistance gene (E. coli)3 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Kras
    MGI:96680    		Kirsten rat sarcoma viral oncogene homolog6Kras<tm1Mok>
    MGI:1857551    		targeted mutation 1, Motoya Katsuki
  • acute myeloid leukemia(MedGEN)

  • arteriovenous malformations of the brain(MedGEN)

  • autoimmune lymphoproliferative syndrome type 4(MedGEN)
    • more 12 Diseases
  • cardiofaciocutaneous syndrome 2(MedGEN)

  • cardiofaciocutaneous syndrome(DisGeNET)

  • exocrine pancreatic carcinoma(MedGEN)

  • gastric cancer(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • juvenile myelomonocytic leukemia(DisGeNET)

  • linear nevus sebaceous syndrome(MedGEN)

  • lung cancer(MedGEN)

  • Noonan syndrome 3(DisGeNET, MedGEN)

  • Noonan syndrome(DisGeNET)

  • Toriello-Lacassie-Droste syndrome(MedGEN)

  • urinary bladder cancer(MedGEN)
    • Hras
    MGI:96224    		Harvey rat sarcoma virus oncogene7Hras<tm1Mok>
    MGI:2386128    		targeted mutation 1, Motoya Katsuki
  • Costello syndrome(DisGeNET, MedGEN)

  • large congenital melanocytic nevus(MedGEN)

  • linear nevus sebaceous syndrome(MedGEN)
    • more 4 Diseases
  • melanoma(DisGeNET)

  • nevus, epidermal(MedGEN)

  • thyroid cancer, nonmedullary, 2(MedGEN)

  • urinary bladder cancer(MedGEN)
    • neo neomycin resistance gene (E. coli)7
    HRAS Harvey rat sarcoma virus oncogene (human)UNHRAS
    loxP phage P1 loxPUNloxP
    loxP phage P1 loxPUNloxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal fetal cardiomyocyte proliferation(MP:0003567)

  • abnormal heart morphology(MP:0000266)

  • chylous ascites(MP:0012080)

  • decreased embryo size(MP:0001698)

  • decreased incidence of tumors by chemical induction(MP:0004502)
    • more 8 phenotypes
  • embryonic growth retardation(MP:0003984)

  • hematopoietic system phenotype(MP:0005397)

  • increased neuron apoptosis(MP:0003203)

  • liver/biliary system phenotype(MP:0005370)

  • prenatal lethality, complete penetrance(MP:0011091)

  • small heart(MP:0002188)

  • thin myocardium(MP:0002652)

  • thin ventricular wall(MP:0000280)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, mouse N-ras genomic DNA, mouse H-ras genomic DNA, mouse K-ras genomic DNA, human H-ras genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 19, 2951-2956 (2000). EMBO J., 21, 1021-1030 (2000). Oncogene, 15, 1151-1159 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data