Strain Data Sheet

RBRC02463

Strain Information

Image
BRC No.RBRC02463
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Nefh<tm1(Venus)Ito>
Former Common nameNF-H KO mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development開発者／機関：神吉浩明先生、糸原重美先生／理研脳センター開発年：2003年
Strain descriptionNF-H遺伝子座Exon1のNotI siteにVenus遺伝子を挿入してある。蛍光蛋白質Venusの発現は検出されない。選択マーカー (pgk-neo) は除去済みである。C57BL/6 isogene.
Colony maintenance繁殖効率良好
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nefhneurofilament, heavy polypeptide11Nefh<tm1(Venus)Ito>targeted mutation 1 (venus), Shigeyoshi ItoharamKIAA0845, NEFH, NF-H, NF200, Nfh

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)11VenusVenus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
myc-tagmyc-epitope tag (rat)11myc-tag

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)11nls

Ordering Information

供与核酸Phage P1 LoxP site, Jellyfish Venus cDNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), yeast FRT site, rat Myc-epitope, bovine BGH poly A, mouse Nefh genomic DNA
Research applicationNeurobiology Research
提供条件条件を付加しない。条件を付加する。
研究成果の公表にあたってVenusの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。Nat. Biotechnol., 20, 87-90 (2002).
Depositor糸原　重美（独立行政法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data