Strain Data Sheet

RBRC02432

Strain Information

Image
BRC No.RBRC02432
TypeTransgeneCartagena
SpeciesMus musculus
Strain name(hRN8-12 x hAG2-5)F1
Former Common nameつくば高血圧マウス
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development筑波大学・深水昭吉（1991）。C57BL/6背景。
Strain descriptionヒトレニン遺伝子のトランスジェニックマウス (hRN8-12) の雌とヒトアンジオテンシノーゲン遺伝子のトランスジェニックマウス (hAG 2-5) の雄を親に持つダブルトランスジェニックマウス。高血圧症を呈する (THM, Tsukuba Hypertensive Mice) 。レニンとアンジオテンシノーゲンはヒト血管を収縮させ高血圧を維持する因子であるレニンーアンジオテンシン系のコンポーネント。腎臓から分泌されるレニンがアンジオテンシノーゲンに働き、アンジオテンシンIが生産され、さらに肝臓や各組織に含まれる変換酵素の作用を受けて強力な血管収縮作用をもつアンジオテンシンIIに変換することが知られている。hRN8-12の雌マウスとhAG 2-5マウスの雄を親に持つダブルトランスジェニックマウス ((hRN8-12 x hAG2-5)F1マウス) は高血圧症を呈する。一方、hAG 2-5の雌マウスにhRN8-12の雄マウスを交配させると、妊娠後期に顕著な血圧上昇認められる (PAH, Pregnancy-Associated Hypertensive) 。hRN8-12マウス (RBRC01122) 、hAG 2-5マウス (RBRC01123) 、hRN8-12 x hAG2-5)F1 (RBRC02432) 。
Colony maintenance
References
Tissue-specific expression of the human renin gene in transgenic mice.
A Fukamizu, M S Seo, T Hatae, M Yokoyama, T Nomura, M Katsuki, K Murakami
Biochem. Biophys. Res. Commun., 165, 826-832 (1989). 2480785

Expression of the human angiotensinogen gene in transgenic mice and transfected cells.
S Takahashi, A Fukamizu, T Hasegawa, M Yokoyama, T Nomura, M Katsuki, K Murakami
Biochem. Biophys. Res. Commun., 180, 1103-1109 (1991). 1659395

Health Report

Examination Date / Room / Rack2024/09/09Room:3-2Rack:C囮検査

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
AGT Angiotensinogen (human)UNAGT
REN1 renin 1 structural (human)UNREN1

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal chorion morphology (MP:0002836)

  • abnormal maternal decidual layer morphology (MP:0004256)

  • abnormal placenta morphology (MP:0001711)

  • abnormal pregnancy (MP:0009661)

  • abnormal renal glomerulus morphology (MP:0005325)
    • more 16 phenotypes
  • abnormal spongiotrophoblast cell morphology (MP:0008959)

  • cardiac fibrosis (MP:0003141)

  • convulsive seizures (MP:0000947)

  • decreased cardiac muscle contractility (MP:0005140)

  • decreased fetal weight (MP:0009431)

  • decreased frequency of paradoxical sleep (MP:0012286)

  • delayed parturition (MP:0012008)

  • heart inflammation (MP:0001853)

  • hemorrhage (MP:0001914)

  • hypertension (MP:0000231)

  • increased heart weight (MP:0002833)

  • increased systemic arterial systolic blood pressure (MP:0006144)

  • increased urine protein level (MP:0002962)

  • maternal effect (MP:0003718)

  • myocardium hypertrophy (MP:0010633)

  • pregnancy-related premature death (MP:0008028)
    • Detailed phenotype data

    Ordering Information

    供与核酸human angiotensinogen genomic DNA ,human renin1 genomic DNA (10 exons, 9 introns and native promoter)
    Research applicationCell Biology Research
    Hematological Research
    Mouse Models for Human Disease
    covid19_progression
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem. Biophys. Res. Commun., 165, 826-832 (1989).
    Biochem. Biophys. Res. Commun., 180, 1103-1109 (1991).
    Depositor深水　昭吉（国立大学法人筑波大学）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month May 2012
    Systolic blood pressure

    BRC mice in Publications

    Hashimoto A, Takeda Y, Karashima S, Kometani M, Aono D, Demura M, Higashitani T, Konishi S, Yoneda T, Takeda Y.
    Impact of mineralocorticoid receptor blockade with direct renin inhibition in angiotensin II-dependent hypertensive mice.
    Hypertens Res 43(10) 1099-1104(2020) 32398797
    Yamagishi K, Tsukamoto I, Nakamura F, Hashimoto K, Ohtani K, Akagi M.
    Activation of the renin-angiotensin system in mice aggravates mechanical loading-induced knee osteoarthritis.
    Eur J Histochem 62(3) (2018) 30043596
    Ito R, Sato I, Tsujita T, Yokoyama A, Sugawara A.
    A ubiquitin-proteasome inhibitor bortezomib suppresses the expression of CYP11B2, a key enzyme of aldosterone synthesis.
    Biochem Biophys Res Commun 489(1) 21-28(2017) 28533088
    Suzuki D, Saito-Hakoda A, Ito R, Shimizu K, Parvin R, Shimada H, Noro E, Suzuki S, Fujiwara I, Kagechika H, Rainey WE, Kure S, Ito S, Yokoyama A, Sugawara A.
    Suppressive effects of RXR agonist PA024 on adrenal CYP11B2 expression, aldosterone secretion and blood pressure.
    PLoS One 12(8) e0181055(2017) 28800627
    Inui Y, Mochida H, Yamairi F, Okada M, Ishida J, Fukamizu A, Arakawa K.
    Effects of aging and uninephrectomy on renal changes in Tsukuba hypertensive mice.
    Biomed Rep 1(3) 359-364(2013) 24648949
    Kawarazaki W, Nagase M, Yoshida S, Takeuchi M, Ishizawa K, Ayuzawa N, Ueda K, Fujita T.
    Angiotensin II- and salt-induced kidney injury through Rac1-mediated mineralocorticoid receptor activation.
    J Am Soc Nephrol 23(6) 997-1007(2012) 22440899