Strain Data Sheet

RBRC02428

Strain Information

Image
BRC No.RBRC02428
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Kras<tm1Mok> Trp53<tm2Mok> Tg(hKrasPAC)1Mok
Former Common namemouse K-ras, p53 KO, K-ras(PAC)Tg, B6-Kras KO/p53 KO/KrasPAC Tg
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences.
Strain descriptionTriple mutant mice having a Kras gene knockout, p53 (Trp53) knockout (lacZ reporter) and human KRAS transgenene.
Colony maintenance
References
Gene replacement of the p53 gene with the lacZ gene in mouse embryonic stem cells and mice by using two steps of homologous recombination.
Gondo Y, Nakamura K, Nakao K, Sasaoka T, Ito K, Kimura M, Katsuki M
Biochem. Biophys. Res. Commun., 1994 202(2):830-7. 8048955

K-ras is essential for the development of the mouse embryo.
Koera K, Nakamura K, Nakao K, Miyoshi J, Toyoshima K, Hatta T, Otani H, Aiba A, Katsuki M
Oncogene, 15, 1151-1159 (1997). 9294608

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trp53
MGI:98834		transformation related protein 5311Trp53<tm2Mok>
MGI:2388704		targeted mutation 2, Motoya Katsuki
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
    • more 14 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)
    • lacZ beta-galactosidase (E. coli)11
    neo neomycin resistance gene (E. coli)11
    Kras
    MGI:96680    		Kirsten rat sarcoma viral oncogene homolog6Kras
  • acute myeloid leukemia(MedGEN)

  • arteriovenous malformations of the brain(MedGEN)

  • autoimmune lymphoproliferative syndrome type 4(MedGEN)
    • more 12 Diseases
  • cardiofaciocutaneous syndrome 2(MedGEN)

  • cardiofaciocutaneous syndrome(DisGeNET)

  • exocrine pancreatic carcinoma(MedGEN)

  • gastric cancer(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • juvenile myelomonocytic leukemia(DisGeNET)

  • linear nevus sebaceous syndrome(MedGEN)

  • lung cancer(MedGEN)

  • Noonan syndrome 3(DisGeNET, MedGEN)

  • Noonan syndrome(DisGeNET)

  • Toriello-Lacassie-Droste syndrome(MedGEN)

  • urinary bladder cancer(MedGEN)
    • KRAS v-Ki-ras2 Kirsten rat sacroma viral oncogene homolog (human)UNKRAS

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal fetal cardiomyocyte proliferation(MP:0003567)

  • abnormal heart morphology(MP:0000266)

  • decreased cellular sensitivity to ultraviolet irradiation(MP:0008411)

  • decreased embryo size(MP:0001698)

  • embryonic growth retardation(MP:0003984)
    • more 8 phenotypes
  • hematopoietic system phenotype(MP:0005397)

  • increased neuron apoptosis(MP:0003203)

  • increased tumor incidence(MP:0002020)

  • liver/biliary system phenotype(MP:0005370)

  • prenatal lethality, complete penetrance(MP:0011091)

  • small heart(MP:0002188)

  • thin myocardium(MP:0002652)

  • thin ventricular wall(MP:0000280)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE.coli neo, mouse K-ras, mouse p53, human K-ras oncogene genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Immunology and Inflammation Research
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 15, 1151-1159 (1997). Biochem. Biophys. Res. Commun., 202, 830-837 (1994). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data