Strain Data Sheet

RBRC02416

Strain Information

Image
BRC No.RBRC02416
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(TRE-GFP)622Yasu
Former Common nameβ-catenin/Tcf reporter mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yasuhiro Yamada, Gifu University Graduate School of Medicine in 2007. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. Line 392 (main line) (RBRC02415), line 622 (rbrc02416) and line 628 (RBRC02417).
Strain descriptionBeta-catenin/Tcf reporter mice. These mice express GFP gene under the control of the Tcf/Lef binding sites and a minimal thymidine kinase promoter from the TOPFLASH plasmid. Immunohistochemistry with a GFP antibody can be used to assess the Tg expression in the intestine. In the splenocytes, expression of GFP was observed by FACS analysis, In other organs, the GFP expression patterns are not examined yet.
Colony maintenance
References
Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Tomita H, Yamada Y, Oyama T, Hata K, Hirose Y, Hara A, Kunisada T, Sugiyama Y, Adachi Y, Linhart H, Mori H
Cancer Res., 67, 4079-4087 (2007). 17483318

Further upregulation of beta-catenin/Tcf transcription is involved in the development of macroscopic tumors in the colon of ApcMin/+ mice.
Oyama T, Yamada Y, Hata K, Tomita H, Hirata A, Sheng H, Hara A, Aoki H, Kunisada T, Yamashita S, Mori H
Carcinogenesis, 29, 666-672 (2008). 18204079

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNTcf binding element, herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNATcf/Lef binding site (TOPFLASH plasmid, Upstate Biotechnology), HSV TK promoter, jellyfish EGFP cDNA, SV40 intron, polyA
    Research applicationCancer Research
    Cell Biology Research
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    General Purpose
    Tet system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
    DepositorYasuhiro Yamada (Gifu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data