Strain Data Sheet

RBRC02415

Information1

Image
BRC No.RBRC02415
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(TRE-GFP)392Yasu
Former Common nameβ-catenin/Tcf reporter mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yasuhiro Yamada, Gifu University Graduate School of Medicine in 2007. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. Line 392 (main line) (RBRC02415), line 622 (rbrc02416) and line 628 (RBRC02417).
Strain descriptionBeta-catenin/Tcf reporter mice. These mice express GFP gene under the control of the Tcf/Lef binding sites and a minimal thymidine kinase promoter from the TOPFLASH plasmid. Immunohistochemistry with a GFP antibody can be used to assess the Tg expression in the intestine. In other organs except for the intestine. In the splenocytes, expression of GFP was observed by FACS analysis, In other organs, the GFP expression patterns are not examined yet.
Colony maintenance
ReferencesCarcinogenesis, 29, 666-672 (2008). 18204079
Cancer Res., 67, 4079-4087 (2007). 17483318

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNTcf binding element, herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Information2

Donor DNATcf/Lef binding site (TOPFLASH plasmid, Upstate Biotechnology), HSV TK promoter, jellyfish EGFP cDNA, SV40 intron, polyA
Research applicationCancer Research
Cell Biology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
General Purpose
Tet system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
DepositorYasuhiro Yamada (Gifu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data