Strain Data Sheet

RBRC02411

Strain Information

Image
BRC No.RBRC02411
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Ptn<tm1Tmu>
Former Common namePtn-KO/C57BL/6J
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Muramatsu H. and Muramatsu T., Nagoya University in 2001.
Strain descriptionPleiotrophin (PTN) knock-out mice, Ptn is a low molecular weight protein that regulates cell growth, survival and migration and other activities of responding cells. Ptn gene strongly expresses in the brain at the later mouse embryogenesis, In the human, it is known that the expression of Ptn gene is observed at the ischemic injury in various cancer.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJmsSlc)
References
Midkine and pleiotrophin: two related proteins involved in development, survival, inflammation and tumorigenesis.
Takashi Muramatsu
J. Biochem., 132, 359-371 (2002). 12204104

The role of midkine and pleiotrophin in liver regeneration.
Keiko Ochiai, Hisako Muramatsu, Satoshi Yamamoto, Hisami Ando, Takashi Muramatsu
Liver Int., 24, 484-491 (2004). 15482347

Female infertility in mice deficient in midkine and pleiotrophin, which form a distinct family of growth factors.
Hisako Muramatsu, Peng Zou, Nobuyuki Kurosawa, Keiko Ichihara-Tanaka, Kiyoko Maruyama, Kazuhiko Inoh, Takayuki Sakai, Lan Chen, Masahiro Sato, Takashi Muramatsu
Genes Cells, 11, 1405-1417 (2006). 17121547

Mice doubly deficient in the midkine and pleiotrophin genes exhibit deficits in the expression of beta-tectorin gene and in auditory response.
Peng Zou, Hisako Muramatsu, Michihiko Sone, Hideo Hayashi, Tsutomu Nakashima, Takashi Muramatsu
Lab. Invest., 86, 645-653 (2006). 16619002

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ptn
MGI:97804		pleiotrophin6Ptn<tm1Tmu>
MGI:3053747		targeted mutation 1, Takashi Muramatsu
neo neomycin resistance gene (E. coli)6 polyoma virus thymidine kinase gene promoter (tk promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal circulating enzyme level (MP:0001570)

  • abnormal hearing physiology (MP:0001963)

  • decreased liver weight (MP:0003402)

  • decreased macrophage cell number (MP:0003884)

  • impaired macrophage chemotaxis (MP:0003799)
    • more 1 phenotypes
  • impaired neutrophil recruitment (MP:0008719)
    • Detailed phenotype data

    Ordering Information

    Donor DNApolyoma virus thymidine kinase gene promoter, E. coli neomycin resistance gene, mouse Ptn genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Liver Int., 24, 484-491 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Hisako Muramatsu) is requested.
    DepositorHisako Muramatsu (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data