Strain Data Sheet

RBRC02388

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC02388
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129P2-Ly96<tm1Kmiy>
Former Common name	C57BL/6-MD-2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development	開発者／機関：三宅健介先生／佐賀医科大学開発年：2002年C57BL/6 Cr, 8回戻し交配済み。
Strain description	リポ多糖 (LPS) に対して不応答性
Colony maintenance	兄妹交配：KOどうしでの交配可能繁殖成績が低い
References	Nat. Immunol. 3, 667-672 (2002) 12055629

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ly96lymphocyte antigen 961Ly96<tm1Kmiy>targeted mutation 1, Kensuke MiyakeESOP-1, MD-2, MD2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information
供与核酸	mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Ly96 genome DNA
Research application	Immunology and Inflammation Research
提供条件	条件を付加する。研究成果の公表にあたって寄託者の指定する文献を引用する。Nature Immunology, 3, 667-672 (2002).
Depositor	三宅　健介（国立大学法人東京大学）
Strain Status	凍結胚凍結精子
Strain Availability	凍結胚より作出したマウスを2~4ヶ月以内に提供可能凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP (Japanese) Genotyping protocol -PCR-

BRC mice in Publications

Fang Q, Wang J, Zhang Y, Wang L, Li W, Han J, Huang W, Liang G, Wang Y. Inhibition of myeloid differentiation factor-2 attenuates obesity-induced cardiomyopathy and fibrosis. Biochim Biophys Acta Mol Basis Dis 1864(1) 252-262(2018) 28965884 Wang M, Zhang Q, Lou S, Jin L, Wu G, Wu W, Tang Q, Wang Y, Long X, Huang P, Luo W, Liang G. Inhibition of MD2 by natural product-drived JM-9 attenuates renal inflammation and diabetic nephropathy in mice. Biomed Pharmacother 168 115660(2023) 37806092 Wang Y, Fang Q, Jin Y, Liu Z, Zou C, Yu W, Li W, Shan X, Chen R, Khan Z, Liang G. Blockade of myeloid differentiation 2 attenuates diabetic nephropathy by reducing activation of the renin-angiotensin system in mouse kidneys. Br J Pharmacol 176(14) 2642-2657(2019) 30959575 Wang Y, Shan X, Chen G, Jiang L, Wang Z, Fang Q, Liu X, Wang J, Zhang Y, Wu W, Liang G. MD-2 as the target of a novel small molecule, L6H21, in the attenuation of LPS-induced inflammatory response and sepsis. Br J Pharmacol 172(17) 4391-405(2015) 26076332 Luo W, Ye L, Hu XT, Wang MH, Wang MX, Jin LM, Xiao ZX, Qian JC, Wang Y, Zuo W, Huang LJ, Liang G. MD2 deficiency prevents high-fat diet-induced AMPK suppression and lipid accumulation through regulating TBK1 in non-alcoholic fatty liver disease. Clin Transl Med 12(3) e777(2022) 35343085 Rajamanickam V, Yan T, Xu S, Hui J, Xu X, Ren L, Liu Z, Liang G, Wang O, Wang Y. Selective targeting of the TLR4 co-receptor, MD2, prevents colon cancer growth and lung metastasis. Int J Biol Sci 16(8) 1288-1302(2020) 32210720 Zhang Y, Wu B, Zhang H, Ge X, Ying S, Hu M, Li W, Huang Y, Wang L, Chen C, Shan X, Liang G. Inhibition of MD2-dependent inflammation attenuates the progression of non-alcoholic fatty liver disease. J Cell Mol Med 22(2) 936-947(2018) 29077272 Fang Q, Wang L, Yang D, Chen X, Shan X, Zhang Y, Lum H, Wang J, Zhong P, Liang G, Wang Y. Blockade of myeloid differentiation protein 2 prevents obesity-induced inflammation and nephropathy. J Cell Mol Med 21(12) 3776-3786(2017) 28767204 Wang Y, Luo W, Han J, Khan ZA, Fang Q, Jin Y, Chen X, Zhang Y, Wang M, Qian J, Huang W, Lum H, Wu G, Liang G. MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy. Nat Commun 11(1) 2148(2020) 32358497 Wang L, Han J, Shan P, You S, Chen X, Jin Y, Wang J, Huang W, Wang Y, Liang G. MD2 Blockage Protects Obesity-Induced Vascular Remodeling via Activating AMPK/Nrf2. Obesity (Silver Spring) 25(9) 1532-1539(2017) 28726347 Chen H, Zhang Y, Zhang W, Liu H, Sun C, Zhang B, Bai B, Wu D, Xiao Z, Lum H, Zhou J, Chen R, Liang G. Inhibition of myeloid differentiation factor 2 by baicalein protects against acute lung injury. Phytomedicine 63 152997(2019) 31254764 Chen H, Song Z, Ying S, Yang X, Wu W, Tan Q, Ju X, Wu W, Zhang X, Qu J, Wang Y. Myeloid differentiation protein 2 induced retinal ischemia reperfusion injury via upregulation of ROS through a TLR4-NOX4 pathway. Toxicol Lett 282 109-120(2018) 29111459

BRC mice in Publications

Fang Q, Wang J, Zhang Y, Wang L, Li W, Han J, Huang W, Liang G, Wang Y.
Inhibition of myeloid differentiation factor-2 attenuates obesity-induced cardiomyopathy and fibrosis.
Biochim Biophys Acta Mol Basis Dis 1864(1) 252-262(2018) 28965884

Wang M, Zhang Q, Lou S, Jin L, Wu G, Wu W, Tang Q, Wang Y, Long X, Huang P, Luo W, Liang G.
Inhibition of MD2 by natural product-drived JM-9 attenuates renal inflammation and diabetic nephropathy in mice.
Biomed Pharmacother 168 115660(2023) 37806092

Wang Y, Fang Q, Jin Y, Liu Z, Zou C, Yu W, Li W, Shan X, Chen R, Khan Z, Liang G.
Blockade of myeloid differentiation 2 attenuates diabetic nephropathy by reducing activation of the renin-angiotensin system in mouse kidneys.
Br J Pharmacol 176(14) 2642-2657(2019) 30959575

Wang Y, Shan X, Chen G, Jiang L, Wang Z, Fang Q, Liu X, Wang J, Zhang Y, Wu W, Liang G.
MD-2 as the target of a novel small molecule, L6H21, in the attenuation of LPS-induced inflammatory response and sepsis.
Br J Pharmacol 172(17) 4391-405(2015) 26076332

Luo W, Ye L, Hu XT, Wang MH, Wang MX, Jin LM, Xiao ZX, Qian JC, Wang Y, Zuo W, Huang LJ, Liang G.
MD2 deficiency prevents high-fat diet-induced AMPK suppression and lipid accumulation through regulating TBK1 in non-alcoholic fatty liver disease.
Clin Transl Med 12(3) e777(2022) 35343085

Rajamanickam V, Yan T, Xu S, Hui J, Xu X, Ren L, Liu Z, Liang G, Wang O, Wang Y.
Selective targeting of the TLR4 co-receptor, MD2, prevents colon cancer growth and lung metastasis.
Int J Biol Sci 16(8) 1288-1302(2020) 32210720

Zhang Y, Wu B, Zhang H, Ge X, Ying S, Hu M, Li W, Huang Y, Wang L, Chen C, Shan X, Liang G.
Inhibition of MD2-dependent inflammation attenuates the progression of non-alcoholic fatty liver disease.
J Cell Mol Med 22(2) 936-947(2018) 29077272

Fang Q, Wang L, Yang D, Chen X, Shan X, Zhang Y, Lum H, Wang J, Zhong P, Liang G, Wang Y.
Blockade of myeloid differentiation protein 2 prevents obesity-induced inflammation and nephropathy.
J Cell Mol Med 21(12) 3776-3786(2017) 28767204

Wang Y, Luo W, Han J, Khan ZA, Fang Q, Jin Y, Chen X, Zhang Y, Wang M, Qian J, Huang W, Lum H, Wu G, Liang G.
MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy.
Nat Commun 11(1) 2148(2020) 32358497

Wang L, Han J, Shan P, You S, Chen X, Jin Y, Wang J, Huang W, Wang Y, Liang G.
MD2 Blockage Protects Obesity-Induced Vascular Remodeling via Activating AMPK/Nrf2.
Obesity (Silver Spring) 25(9) 1532-1539(2017) 28726347

Chen H, Zhang Y, Zhang W, Liu H, Sun C, Zhang B, Bai B, Wu D, Xiao Z, Lum H, Zhou J, Chen R, Liang G.
Inhibition of myeloid differentiation factor 2 by baicalein protects against acute lung injury.
Phytomedicine 63 152997(2019) 31254764

Chen H, Song Z, Ying S, Yang X, Wu W, Tan Q, Ju X, Wu W, Zhang X, Qu J, Wang Y.
Myeloid differentiation protein 2 induced retinal ischemia reperfusion injury via upregulation of ROS through a TLR4-NOX4 pathway.
Toxicol Lett 282 109-120(2018) 29111459

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications