Strain Data Sheet

RBRC02351

Strain Information

Image
BRC No.RBRC02351
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Slc1a3<tm1(cre)Ksak>
Former Common nameGLAST-Cre
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain development新潟大学脳研究所・辻田実加、夏目里恵、崎村建司（2004）。C57BL/6NCrj由来のRENKA ES細胞株を用いて作製。C57BL/6N背景。
Strain descriptionグルタミン酸トランスポーター遺伝子Slc1a3のCreノックインマウス。Slc1a3(GLAST)遺伝子の開始メチオニンからインフレームでCre配列を挿入。Creの下流にG-CSF3’ non-cordingとpgk-neoカセットをもつ。pgk-neoカセットは両側にfrt配列をもち、FLPリコンビネースにより除去が可能。
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc1a3solute carrier family 1 (glial high affinity glutamate transporter), member 315Slc1a3<tm1(cre)Ksak>targeted mutation 1, Kenji SakimuraB430115D02Rik, Eaat1, GLAST, Gmt1, MGluT1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase15cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Amouse pgk polyA15poly A

Ordering Information

供与核酸Bacteriophage P1 cre, yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK poly A, S. cerevisiae 2m plasmid, mouse Slc1a3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
General Purpose
Neurobiology Research
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-auther in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
Depositor崎村　建司（国立大学法人新潟大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data