Strain Data Sheet

RBRC02351

Information1

Image
BRC No.RBRC02351
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Slc1a3<tm1(cre)Ksak>
Former Common nameGLAST-Cre
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Mika Tsujita, Rie Natsume and Kenji Sakimura, Brain Research Institute, Niigata University in 2004. RENKA ES cells derived from C57BL/6NCrj were used. C57BL/6N background.
Strain descriptionSlc1a3 Cre knock-in mice. A Cre sequence was integrated into the start methionine site of the Slc1a3 (GLAST) gene at an in-frame position. A non-coding and pgk-neo cassette sequence are located downstream from Cre. The pgk-neo cassette can be removed, as it has an frt sequence on both sides.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc1a3Progesterone receptor15Slc1a3<tm1(cre)Ksak>targeted mutation 1, Kenji SakimuraB430115D02Rik, Eaat1, GLAST, Gmt1, MGluT1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase15cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Amouse pgk polyA15poly A

Information2

Donor DNABacteriophage P1 cre, yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK poly A, S. cerevisiae 2m plasmid, mouse Slc1a3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
General Purpose
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-auther in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorKenji Sakimura (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data