Strain Information | |
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Image | |
BRC No. | RBRC02350 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Slc1a3<tm1(crePR)Ksak> |
Former Common name | GLAST-CrePR |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | C57BL/6NCrlCrlj |
Appearance | |
Strain development | 新潟大学脳研究所・辻田実加、夏目里恵、崎村建司(2004)。C57BL/6NCrj由来のRENKA ES細胞株を用いて作製。C57BL/6N背景。 |
Strain description | グルタミン酸トランスポーター遺伝子Slc1a3のCrePRノックインマウス。Slc1a3 (GLAST)遺伝子の開始メチオニンからインフレームでCrePR配列を挿入。CrePRの下流にG-CSF3’ non-cordingとpgk-neoカセットをもつ。pgk-neoカセットは両側にfrt配列をもち、FLPリコンビネースにより除去が可能。 |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrlCrlj] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Slc1a3solute carrier family 1 (glial high affinity glutamate transporter), member 315Slc1a3<tm1(crePR)Ksak>targeted mutation 1, Kenji SakimuraB430115D02Rik, Eaat1, GLAST, Gmt1, MGluT1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PGRProgesterone receptor (human)15PGR Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase15cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)15neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly Amouse pgk polyA15poly A |
Ordering Information | |
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供与核酸 | Bacteriophage P1 Cre, Human progesterone receptor cDNA, S. cerevisiae 2m plasmid, yeast FRT (flippase recombination target) sites, mouse phosphoglycerokinase(pgk) promoter, E. coli neo, mouse pgk poly A, mouse Slc1a3 genomic DNA |
Research application | Cre/loxP system FLP/frt system General Purpose Neurobiology Research |
提供条件 | 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-auther in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
Depositor | 崎村 建司(国立大学法人新潟大学) |
Strain Status | 凍結胚 凍結精子 |
Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |