Strain Data Sheet

RBRC02340

Strain Information

Image
BRC No.RBRC02340
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(bitetO-GFP)BYoko
Former Common namebitetO-GFP
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development開発者／機関名：横井峰人先生／コロンビア大学開発年：2002年
Strain descriptionテトラサイクリン依存性プロモーターにより発現調節を受け、細胞全体が緑色蛍光にて可視化できるトランスジェニックマウス。
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]Tg/+ hemizygous mice are viable and fertile.
ReferencesJ. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gria2glutamate receptor, ionotropic, AMPA2 (alpha 2)UNGria2Glur-2, GluR-B, GluR2, Glur2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptprdprotein tyrosine phosphatase, receptor type, DUNPtprd1110002J03Rik, 3000002J10Rik, B230219D21Rik, MGC:36851

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNE. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin intron/polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PLAPhuman placental alkaline phosphataseUNPLAP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TTCnon-toxic c fragment (Clostridium tetani)UNTTC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ASV40 polyA signalUNpoly A

Ordering Information

供与核酸E. coli tetO, CMV minimal promoter, Aequoria Victoria GFP cDNA, Rabbit beta-Globin intron/polyA signal, Human placental alkaline phosphatase, Clostridium tetani Non-toxic c fragment, Mouse tyrosine phosphatase δ(fragment), Mouse glutamate receptor 2 (fragment), SV40 polyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Tet system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurosci., 27, 6948-6955 (2007).
公表を前提とした非営利目的の学術研究での使用に限る。
Depositor横井　峰人（国立大学法人京都大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Yamada M, Suzuki Y, Nagasaki SC, Okuno H, Imayoshi I.
Light Control of the Tet Gene Expression System in Mammalian Cells.
Cell Rep 25(2) 487-500.e6(2018) 30304687