Strain Data Sheet

RBRC02340

Information1

Image
BRC No.RBRC02340
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(bitetO-GFP)BYoko
Former Common namebitetO-GFP
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Columbia University in 2002. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionThis transgenic mice express a bitetO-GFP contained the bidirectional tetracycline operator (tetO) promoter driving EGFP and a rabbit beta-globin intron/polyA signal in one direction, and in the opposite direction, driving an artifical intron, an artificial fusion gene consisting of a placental alkaline phosphatase fragment, tetanus toxin C fragment, tyrosine phosphatase delta fragment, and glutamate receptor 2 fragment (RT8) and an SV40 polyadenylation signal. This strain can be used to make an inducible expression of EGFP crossing with the genetically-engineered mice that have a tetracycline-controlled transactivator (tTA).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]Tg/+ hemizygous mice are viable and fertile.
ReferencesJ. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack2010/11/16Room:4-ARack:E
2010/09/13Room:4-ARack:E
2010/07/12Room:4-ARack:E
2010/05/17Room:4-ARack:E
2010/03/15Room:4-ARack:E
2010/01/18Room:4-ARack:E
2009/11/16Room:4-ARack:E
2009/09/14Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gria2glutamate receptor, ionotropic, AMPA2 (alpha 2)UNGria2Glur-2, GluR-B, GluR2, Glur2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptprdprotein tyrosine phosphatase, receptor type, DUNPtprd1110002J03Rik, 3000002J10Rik, B230219D21Rik, MGC:36851

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNE. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin intron/polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PLAPhuman placental alkaline phosphataseUNPLAP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TTCnon-toxic c fragment (Clostridium tetani)UNTTC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ASV40 polyA signalUNpoly A

Information2

Donor DNAE. coli tetO, CMV minimal promoter, Aequoria Victoria GFP cDNA, Rabbit beta-Globin intron/polyA signal, Human placental alkaline phosphatase, Clostridium tetani Non-toxic c fragment, Mouse tyrosine phosphatase δ(fragment), Mouse glutamate receptor 2 (fragment), SV40 polyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 27, 6948-6955 (2007).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
DepositorYokoi Mineto (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

Mayumi Yamada, Yusuke Suzuki, Shinji C.Nagasaki, Hiroyuki Okuno, Itaru Imayoshi.
Light Control of the Tet Gene Expression System in Mammalian Cells.
Cell Rep 25(2) 487-500.(2018) 30304687