Strain Data Sheet
RBRC02338
Strain Information | |
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| Image |  |
| BRC No. | RBRC02338 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129P2-p130Cas<tm2.1Homy> |
| Former Common name | Cas-FL conditional KO |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | black [a/a B/B C/C] |
| Strain development | 開発者／機関：本田浩章先生／広島大学原爆放射線医科学研究所開発年：2005年特記：寄託時の戻し交配数は3。 |
| Strain description | Cas<p130> (Crk-associated sucstrate p130)遺伝子のexon2からexon7のゲノムの位置にCas cDNAのcoding領域をloxPで挟んだものと置換してあるfloxマウス(p130Casの完全なconditional knockout mice) |
| Colony maintenance | 戻し交配 Background系統：C57BL/6N（日本チャールズ・リバー） |
| References | Am. J. Physiol. Cell Physiol., 304(6):C541-7 (2013). 23325412 J. Bone Miner. Res., 28(12):2449-62 (2013). 23526406 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Bcar1breast cancer anti-estrogen resistance 18Bcar1<tm2.1Homy>targeted mutation 2.1, Hiroaki HondaCas, Crkas, p130 Cas Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site8Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP |
Ordering Information | |
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| 供与核酸 | phage P1 loxP sites, rat Cas cDNA, SV40 poly A, yeast FRT site, mouse Cas genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| 提供条件 | 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。 |
| Depositor | 本田 浩章（東京女子医科大学） |
| Strain Status |  凍結胚 凍結精子 |
| Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Akimoto T, Okuhira K, Aizawa K, Wada S, Honda H, Fukubayashi T, Ushida T. Skeletal muscle adaptation in response to mechanical stress in p130cas-/- mice. Am J Physiol Cell Physiol 304(6) C541-7(2013) 23325412 Miyazaki T, Zhao Z, Ichihara Y, Yoshino D, Imamura T, Sawada K, Hayano S, Kamioka H, Mori S, Hirata H, Araki K, Kawauchi K, Shigemoto K, Tanaka S, Bonewald LF, Honda H, Shinohara M, Nagao M, Ogata T, Harada I, Sawada Y. Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity. Sci Adv 5(9) eaau7802(2019) 31579816 |